Vero Host Cell Residue DNA Size Analysis Kit _ 41314ES

SKU: 41314ES70

Size: 4x50 T
価格:
販売価格$4,780.00

送料計算済み チェックアウト時

在庫:
在庫あり

説明

The Vero Host Cell Residue DNA Size Analysis Kit is designed for the quantitative analysis of Vero residual DNA fragments of various lengths in intermediates, semi-finished products, and final products of biological preparations.

This kit utilizes the qPCR fluorescence probe principle to specifically and rapidly detect Vero DNA residues both below and above 200 base pairs, with a quantification limit as low as 10 fg/μL. It also includes the Vero DNA Control (DNA quantitative reference). This kit can be used in conjunction with the company's magnetic bead-based residual DNA sample preparation kits (Cat#18461ES).

Feature

  • Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards;
  • Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory;
  • High sensitivity: the limit of quantification can reach 3fg/μL level;
  • High precision: high intra-batch repeatability and low inter-batch variation;
  • Strong exclusivity: specific detection of residual DNA in Vero cells without interference from other exogenous genomic DNA;
  • Strong anti-interference: add internal control (IC), easy to exclude sample interference, reaction preparation abnormalities and other factors.

Application

Residual Vero Host Cell DNA Size test in biological products

Specification

Sensitivity

3fg/μL (range 300pg/μL~3fg/μL)

Assay Time

~1.5 hours

Assay Principle

Fluorescent probe qPCR method

Required Instruments

Real-time fluorescence quantitative PCR system

Components

Components No.

Name

41314ES70-EN

41314ES74-EN

41314-A

Vero qPCR Mix

0.75 mL×4

1.5 mL×4

41314-B1

Vero Primer&Probe Mix-97

200 μL

400 μL

41314-B2

Vero Primer&Probe Mix-154

200 μL

400 μL

41314-B3

Vero Primer&Probe Mix-219

200 μL

400 μL

41314-B4

Vero Primer&Probe Mix-507

200 μL

400 μL

41314-C

DNA Dilution Buffer

1.8 mL×2

1.8 mL×4

41314-D

Vero DNA Control (30 ng/μL)

25 μL

50 μL

41314-E

IC

200 μL

400 μL

Storage

This product should be stored at -25~-15℃ for 2 years.

Both 41314-A and 41314-B1&B2&B3&B4 all should be stored protected from light.

Figures

  • Limit of quantification

The primer probes of the four fragments, 97bp, 154bp, 219bp and 507bp, were used to detect Vero DNA at 30pg/μL, 300fg/μL, and 3fg/μL, respectively, with 10 replicates for each concentration. The results showed that at concentrations of 3 fg/μL and above, the CVs of the four fragments were <20%, and the backcalculated ratios were between 70% and 130%, so the limit of quantification of the four fragments of the Vero Residual DNA Fragmentation Assay Kit was 3 fg/μL.

 

 

Repetition number

 

Test item

Vero DNA (fg/uL)

97bp

154bp

219bp

507bp

Quantity

Return ratio

Quantity

Return ratio

Quantity

Return ratio

Quantity

Return ratio

1

3.49

116.33

2.48

82.67

3.33

111.00

3.53

117.61

2

3.03

101.00

3.23

107.67

2.62

87.33

3.56

118.65

3

3.64

121.33

3.10

103.33

3.52

117.33

3.34

111.50

4

3.01

100.33

2.55

85.00

3.58

119.33

3.36

112.03

5

2.92

97.33

3.15

105.00

3.53

117.67

2.66

88.64

6

2.96

98.67

2.77

92.33

3.34

111.33

2.93

97.80

7

3.33

111.00

2.88

96.00

3.14

104.67

3.35

111.52

8

2.34

78.00

3.32

110.67

3.01

100.33

2.69

89.79

9

2.84

94.67

3.84

128.00

2.85

95.00

3.33

111.01

10

2.89

96.33

2.88

96.00

3.00

100.00

3.47

115.67

Average value

3.05

\

3.02

\

3.19

\

3.22

\

CV

12.09%

\

13.26%

\

10.07%

\

10.39%

\

Table 1. Detection results of limit of quantification for four fragments of 97bp, 154bp, 219bp and 507bp

Documents:

Safety Data Sheet

Manuals

支払いとセキュリティ

American Express Apple Pay Diners Club Discover Google Pay Mastercard Visa

お支払い情報は安全に処理されます。 クレジットカードの詳細を保存したり、クレジットカード情報にアクセスすることはありません

問い合わせ

あなたも好きかもしれません

よくある質問

この製品は研究目的のみに使用され、人間や動物の治療や診断に使用することを意図したものではありません。製品とコンテンツは、Yeasen Biotechnology が所有する特許、商標、著作権によって保護されています。商標記号は原産国を示しますが、必ずしもすべての地域で登録されているわけではありません。

特定のアプリケーションでは、追加のサードパーティの知的財産権が必要になる場合があります。

Yeasen は倫理的な科学に専念しており、私たちの研究は安全性と倫理基準を確保しながら重要な問題に取り組むべきだと考えています。