The Hieff™ Fast Cell Direct Probe RT-qPCR Kit is designed for quantitative gene expression analysis of various animal cells, including adherent and suspension cell lines, primary cultured cells, various stem cells, and iPS cells.

Eliminating the need for RNA extraction, this kit allows for direct qPCR analysis. It features a simple, low-error workflow that efficiently completes all steps—from template preparation and reverse transcription to gene expression analysis—in as little as 1.5 hours. The kit includes both reverse transcription and quantitative detection reagents, facilitating easy and rapid gene expression analysis.

Features

Complete Workflow Solution: Includes cell lysis reagent, wash buffer, gDNA removal reagent, reverse transcription mix, and probe-based qPCR master mix

Fast & Convenient: Complete RNA extraction, reverse transcription, and qPCR workflow in as little as 1.5 hours

High Sensitivity & Reproducibility: Improved amplification performance across genes with varying GC content and excellent repeatability

Excellent Inhibitor Tolerance: Compatible with up to 55% cell lysate input

Outstanding Stability: Stable for 7 days at 4°C; fully premixed qPCR system remains stable for 48 hours

Components

[Note]: *If the supplied amount is insufficient, the Lysis set (Cat#16814ES), RT set (Cat#16815ES), and qPCR set (Cat#16816ES) are available for separate purchase.

Storage

Store unopened kits (11173-A and 11173-B) at -25 ~ -15°C. Once thawed, store at 4°C and take precautions to prevent contamination. For long-term storage, keep all other components at -25 ~ -15°C. Valid for 1 year.

Figures

1. High Sensitivity

 Figure 1. Comparison of gene expression analysis:

Gene expression analysis using 293 cells in different states as templates. Samples containing 10³, 10⁴, 10⁵, and 10⁶ cells were detected using Yeasen 11173ES and supplier T* respectively. The results demonstrate that Yeasen 11173ES consistently yields lower Ct values than supplier T* across all cell input amounts, indicating superior sensitivity and better reproducibility.

2. High Detection Efficiency

 Figure 2. Performance evaluation: Direct Amplification vs. Traditional RNA Extraction.

Using 293 cells as the template, gene expression levels were detected by both direct lysis and conventional RNA extraction workflows with the reverse transcription and qPCR components included in the kit. The results demonstrated that the direct amplification workflow of 11173ES delivers performance comparable to traditional time-consuming RNA extraction methods, while offering a faster and more efficient solution.

3. Excellent Inhibitor Tolerance

Figure 3. Inhibition Resistance Test

(a) Reverse Transcription (RT) System: The 11173ES RT system tolerated up to 55% cell lysis product, demonstrating robust resistance to inhibition.

(b) qPCR System: The 11173ES qPCR system tolerated up to 46% cDNA input, maintaining efficient amplification even in the presence of high concentrations of lysis-derived cDNA.

4. Excellent Stability

Figure 4. Evaluation of pre-mixed qPCR system stability.

Stability was assessed by incubating the fully pre-mixed qPCR system (containing cDNA, primers, probes, and H₂O) at various temperatures for up to 48 hours. The consistent Ct values indicate that the 11173ES qPCR system is highly stable, offering a robust solution that accommodates flexible experimental schedules and operational needs. 

Documents:

Safety Data Sheet

11173_MSDS_HB260511_EN.PDF

Manuals

11173_Manual_Ver.EN20250511.pdf

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