説明
T7 High Yield RNA Synthesis Kit (low dsRNA) optimizes the transcription reaction system. The T7 RNA polymerase used in this kit is an engineered and optimized variant of wild-type T7 RNA polymerase, obtained by mutation, which can significantly reduce dsRNA content. Using linear double-stranded DNA containing a T7 promoter sequence as template and NTPs as substrates, it transcribes the DNA sequence downstream of the promoter and efficiently synthesizes single-stranded RNA. Modified nucleotides can be incorporated into the substrates during transcription to prepare biotin- or dye-labeled RNA.
This kit can synthesize both long and short transcripts. With 1 μg of template input, 100–200 μg of RNA can be produced. The synthesized RNA can be used for a variety of downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.
Product Information
|
Catalog No. |
Size |
|
10633ES10 / 10633ES50 / 10633ES60 / 10633ES70 |
10 T / 50 T / 100 T / 500 T |
Component Information
|
Component No. |
Component Name |
10633ES10 |
10633ES50 |
10633ES60 |
10633ES70 |
|
10633-A |
T7 RNA Polymerase Mix (low dsRNA), 20 U/μL |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-B |
10× Transcription Buffer |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-C |
ATP (100 mM) |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-D |
CTP (100 mM) |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-E |
GTP (100 mM) |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-F |
UTP (100 mM) |
20 μL |
100 μL |
200 μL |
1 mL |
|
10633-G |
Control DNA Template (500 ng/μL) |
5 μL |
10 μL |
20 μL |
100 μL |
|
10633-H |
DNase I (2 U/μL) |
10 μL |
50 μL |
100 μL |
500 μL |
|
10633-I |
Lithium Chloride Solution |
300 μL |
1.5 mL |
3 mL |
15 mL |
|
10633-J |
RNase-free ddH₂O |
500 μL |
2.5 mL |
5 mL |
25 mL |
Feature
• Extremely high yield—up to 200 μg in 2 hours
• Broad applicability—suitable for transcription of 20 nt to 10,000 nt RNA
• Extremely low dsRNA—significantly reduces dsRNA content during IVT
• Multiple applications—suitable for standard, labeled, and modified RNA synthesis
Applications
1. Under different length applications, both 2K and 4K fragments achieved yields of ~11 mg/mL; with the low dsRNA T7 system, dsRNA content was drastically reduced.
|
Fragment length |
T7 RNA pol |
Yield (mg/mL) |
Integrity (%) |
dsRNA content (ng dsRNA produced from 1 μg RNA) |
|
2K |
T7-WT |
12.4 |
93.4 |
0.01728 |
|
|
T7-low dsRNA |
12.2 |
94.1 |
0.0003 |
|
4K |
T7-WT |
11.4 |
89.3 |
0.4273 |
|
|
T7-low dsRNA |
11.7 |
89.4 |
0.0129 |
2. High integrity
Figure 1: 44–4000 nt RNA analyzed on the Agilent 2100 Bioanalyzer. The electrophoretic bands are single and the peaks are distinct and sharp, indicating high RNA integrity.
Storage
Store at −25°C to −15°C. Shelf life: 1 year.
Documents:
Safety Data Sheet
Manual
支払いとセキュリティ
お支払い情報は安全に処理されます。 クレジットカードの詳細を保存したり、クレジットカード情報にアクセスすることはありません
あなたも好きかもしれません
問い合わせ
よくある質問
この製品は研究目的のみに使用され、人間や動物の治療や診断に使用することを意図したものではありません。製品とコンテンツは、
特定のアプリケーションでは、追加のサードパーティの知的財産権が必要になる場合があります。