Validated protocols optimized for a wide range of cell culture and in vivo applications.
Ceturegel™ Matrix Gel offers a stable extracellular matrix (ECM) environment that closely mimics the physiological conditions of native tissues. These protocols are designed to help researchers achieve reproducible and high-quality results with minimal optimization.
Our standardized methods cover various experimental needs—from traditional 2D culture and advanced 3D organoid systems to invasion, angiogenesis, and in vivo tumor formation assays. Each protocol includes detailed step-by-step procedures, recommended cell densities, handling temperatures, and critical parameters to ensure optimal performance.
All protocols have been validated by leading universities and research institutions, demonstrating high reliability and broad compatibility with different cell types and assay systems.
Optimized Application Protocols for Ceturegel™ Matrix Gel
|
Application |
Description |
Example Cell Type / Model |
Key Features |
|
Coating culture plates with diluted Ceturegel™ Matrix Gel to improve cell attachment, spreading, and physiological morphology. |
Epithelial cells, endothelial cells, stem cells |
Promotes natural adhesion and maintains differentiation potential under physiological stiffness. |
|
|
Embedding cells or organoid fragments within Ceturegel™ Matrix Gel to simulate native ECM conditions. |
Intestinal organoids, tumor spheroids, stem cell-derived organoids |
Enables self-organization, tissue polarity, and long-term culture stability. |
|
|
Forming a 3D matrix barrier using Ceturegel™ Matrix Gel to assess cell migration and invasion capabilities. |
Tumor cells (e.g., MDA-MB-231, A549) |
Reproduces ECM remodeling and invasive behavior under controlled gradients. |
|
|
Using Ceturegel™ Matrix Gel as a scaffold for endothelial tube formation and vascular network modeling. |
HUVECs, microvascular endothelial cells |
Supports rapid capillary-like tube formation and quantitative angiogenesis analysis. |
|
|
Mixing tumor cells with chilled Ceturegel™ Matrix Gel prior to subcutaneous or orthotopic injection in mice. |
Cancer cell lines, patient-derived xenografts (PDXs) |
Enhances cell viability, local retention, and tumor take rate in vivo. |
|
|
Employing Ceturegel™ Matrix Gel as an ECM-mimicking substrate to assess platelet adhesion and clot formation. |
Endothelial or platelet adhesion models |
Provides physiologically relevant matrix stiffness for coagulation and thrombosis research. |