The GoldBand™ 100 bp Plus DNA Ladder consists of fourteen linear double-stranded DNA fragments of 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1,000 bp, 1,200 bp, 1,500 bp, 2,000 bp, and 3,000 bp, stored in 1× DNA Loading Buffer, ready for direct use in electrophoretic analysis. The indicator bands are 500 bp and 1,200 bp, with a concentration of 120 ng/5 μL, while all other bands are 50 ng/5 μL. This product is suitable for the analysis of DNA bands in agarose gel electrophoresis and is not recommended for polyacrylamide gel electrophoresis.

Features

Superior Performance: Clean background, stable bands, and precise sizing.

Semi-Quantitative: Indicator bands with known concentrations for easy analysis.

Ready-to-Load: Pre-mixed with loading buffer for direct electrophoresis.

Room Temperature Stability: Stable for 6 months at RT.

Bonus: Includes 5× loading buffer for your samples.

Components

Storage

Store at room temperature or 2~8°C, valid for 6 months; store at -25~-15°C, valid for 2 years. Avoid repeated freeze-thaw cycles.

Electrophoresis Diagram

Figure 1. Electrophoretic Band Pattern of 100 bp Plus DNA Ladder

Documents:

Safety Data Sheet

10516_MSDS_HB260512_EN.PDF

Manuals

10516_Manual_Ver.EN20250512.pdf

FAQ

Q1: Is the DNA marker an enzyme digest product or a PCR product?

A1: It is a mixture of PCR products.

Q2: How do I choose the right DNA Marker?

A2: Simply ensure that the size of your target band falls within the range of the smallest and largest bands of the DNA Marker for accurate size reference.

Q3: Is this Marker suitable for PAGE gels?

A3: This Marker is designed for agarose gel electrophoresis. It has not been tested in PAGE gels.

Q4: Can I use a DNA marker for RNA electrophoresis?

A4: It depends on the gel type. For denaturing gel electrophoresis, using a DNA Marker is not recommended as the double strands will separate. For standard agarose gel electrophoresis, a DNA Marker is not accurate for determining RNA size, but it is acceptable for a rough estimation.

Q5: The DNA marker bands are not resolving well and appear severely curved/bowed?

A5: Possible causes: a) Voltage is too high; b) YeaRed nucleic acid stain was added when the agarose was still too hot.

Solutions: Adjust the voltage to 110-130V and run for at least 45 minutes. Allow the melted agarose to cool until it is no longer hot to the touch before adding the YeaRed stain.

Q6: The DNA Marker looks good, but my sample bands are curved?

A6: This is likely due to sample overloading.

Solution: Reduce the sample loading volume.

Q7: Why are the small molecular weight bands of the DNA Marker weaker?

A7: This is a normal phenomenon. Cationic (positively charged) nucleic acid stains migrate in the opposite direction to nucleic acids in an electric field. Since small DNA fragments migrate faster and are ahead of the stain, they bind less dye, resulting in weaker fluorescence.