شرح
Agarose is a purified linear galactoglycan hydrophilic colloid derived from agar or agar-containing algae. It is a linear polymer composed of β-d-galactosyl (1-4) linked to 3, 6-dehydrated-L-galactosyl. As a gel reagent, it is commonly used for routine nucleic acid analysis by gel electrophoresis or blotting (such as Northern or Southern) and for protein applications, such as radiative immunodiffusion (RID) assay.
Applications
Gel electrophoresis
Components
|
Name |
10208ES08 |
10208ES60 |
10208ES70 |
|
Agarose |
5 g |
100 g |
500 g |
Specifications
|
9012-36-6 |
|
|
Appearance |
White to off-white powder |
|
Gel Strength (1.0%) |
≥1200 (g/cm2) |
|
Gel Point (1.5%) |
36±1.5℃ |
|
Melting Point (1.5%) |
88±1.5℃ |
|
EEO (-mr) |
≤0.13 |
|
Sulfate (%) |
≤0.15% |
|
Moisture |
≤10% |
|
DNase |
None Detected |
|
RNase |
None Detected |
|
Protease |
None Detected |
Storage
The Agarose products should be stored at room temperature for 5 years.
FAQ
Q: Is the 10208ES Low Melting Point Agarose sterile and nuclease-free? What about other types of agarose?
A: It is sterile and nuclease-free. Low melting point agarose is commonly used for plaque assays, so it can be used safely for cell culture. Other types of agarose are also sterile and nuclease-free.
Q: Why is the 10208ES agarose relatively soft? What is the reason for this?
A: The firmness is related to the gel strength. Agarose is extracted from agar, and there will always be some variation in strength levels; it is impossible for every batch to be identical. The gel strength specification for this agarose is $\ge 1200$, and as long as it is above this range, it is considered qualified.
Q: Why do the bands appear smeared when using 10208ES?
A: The gelling time may have been too short, resulting in an uneven gel. For a 1% agarose gel, the minimum gelling time is 30 minutes.
Q: What size range of bands can high-resolution agarose separate?
A: The resolution for both high-resolution agarose and 3:1 agarose is around 2% (meaning if the fragment size is 30 bp, it can separate $30 \times 0.02$; for a 100 bp fragment, $100 \times 0.02$).
Q: Can 10208ES low melting point agarose be used for pulsed-field gel electrophoresis (PFGE)?
A: No, pulsed-field gel electrophoresis requires very low electroosmosis and very high gel strength.
Documents:
Citations & References:
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[2] Zhang Y, Ding H, Wang X, et al. MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. Cell Rep. 2021;37(5):109949. doi:10.1016/j.celrep.2021.109949(IF:9.423)
[3] Zhu Z, Zhang L, Sheng R, Chen J. Microfluidic-Based Cationic Cholesterol Lipid siRNA Delivery Nanosystem: Highly Efficient In Vitro Gene Silencing and the Intracellular Behavior. Int J Mol Sci. 2022;23(7):3999. Published 2022 Apr 3. doi:10.3390/ijms23073999(IF:5.924)
[4] Zhao C, Yang D, Ye Y, et al. Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP inhibitor in multiple myeloma. Biochem Pharmacol. 2021;190:114648. doi:10.1016/j.bcp.2021.114648(IF:5.858)
[5] Yu J, Yang W, Xing S, et al. Blended gold/MnO<sub>2</sub>@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid. Mikrochim Acta. 2019;186(2):89. Published 2019 Jan 10. doi:10.1007/s00604-018-3205-8(IF:5.479)
[6] Zheng X, Xu W, Sun R, Yin H, Dong C, Zeng H. Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells. Chem Biol Interact. 2017;275:74-85. doi:10.1016/j.cbi.2017.07.020(IF:5.194)
[7] Zhou J, Xiong R, Zhou J, et al. Involvement of m6A regulatory factor IGF2BP1 in malignant transformation of human bronchial epithelial Beas-2B cells induced by tobacco carcinogen NNK. Toxicol Appl Pharmacol. 2022;436:115849. doi:10.1016/j.taap.2021.115849(IF:4.219)
[8] Zhou Y, Liu J, Cai S, Liu D, Jiang R, Wang Y. Protective effects of ginsenoside Rg1 on aging Sca-1⁺ hematopoietic cells. Mol Med Rep. 2015;12(3):3621-3628. doi:10.3892/mmr.2015.3884(IF:2.952)
[9] Zheng SY, Ma LL, Wang XL, et al. RPA-Cas12aDS: A visual and fast molecular diagnostics platform based on RPA-CRISPR-Cas12a method for infectious bursal disease virus detection. J Virol Methods. 2022;304:114523. doi:10.1016/j.jviromet.2022.114523(IF:2.014)
[10] Sun Z, Zhang H, Xie K, Tan X, Zhang H, Chen J. Rice Black-streaked Dwarf Virus Preparation and Infection on Rice. Bio Protoc. 2017;7(24):e2651. Published 2017 Dec 20. doi:10.21769/BioProtoc.2651(IF:0.000)
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