Beschreibung
|
Product Alias |
K-Ras 2,C-K-RAS,KRAS,MHC |
|
Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGADGVGK peptide |
|
Accession |
AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK |
|
Label |
C-His-Avi |
|
Expression system |
E.coli |
|
Molecular weight |
The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【SPR Data】Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR with an affinity constant of 6.93 nM as determined in SPR assay . |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High Purity, High Activity, Low Endotoxin, High Batch-to-Batch Consistency.
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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FAQ
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