Product Alias

sHLA-G,HLA G antigen, b2 microglobulin

Species

Rhesus macaque

Expression sequence

Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide

Accession

O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on SDS-PAGE result.

purity

> 95% as determined by SDS-PAGE and HPLC.

endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA . 【SPR Data】Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 27.18 nM as determined in SPR assay .

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.