Beschreibung
This kit is a DNA enzyme digestion library preparation reagent designed for the third-generation Nanopore sequencing platform. This product uses high-quality fragmentation enzymes, gets rid of the cumbersome ultrasonic process, simplifies the operation process, and combines the fragmentation module with the end repair/addition A module, which greatly reduces the time and cost of library preparation. It can be applied to 50 ng-1 μg of conventional animal and plant genomes, microbial genomes and other samples, and realize DNA fragmentation, end repair and A-tail addition reactions in a single tube.
Features
- Easy to operate: One-tube reaction, DNA fragmentation, end repair and A-tail addition reactions can be performed in a single tube
- Wide compatibility: Applicable to conventional animal and plant genomes, microbial genomes, FFPE and other samples
- Easier operation process: The fragmentation module and the end repair/A-tail addition module are combined into one, reducing the time and cost of library construction
- Compatible with automated equipment: The reagents are in Mix form and are compatible with automated library construction equipment
Specifications
|
Cat.No. |
13309ES08 / 13309ES24 / 13309ES96 |
|
Size |
8 T / 24 T / 96 T |
Components
|
Component No. |
Name |
13309ES08 |
13309ES24 |
13309ES96 |
|
13309-A |
Smearase Buffer |
80 μL |
240 μL |
960 μL |
|
13309-B |
Smearase Enzyme |
40 μL |
120 μL |
480 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for one year.
Figures
A. DNA fragment test
Figure 1. DNA fragment detection of different reaction time
Template: 293 gDNA, input DNA 500 ng. According to the recommended fragmentation time and magnetic bead purification ratio, 4-20 Kb fragments can be obtained.
B. higher long fragment read rate
Documents:
Safety Data Sheet
Manuals
13309_Manual_Ver.EN20250512.pdf
Citations & References:
Zahlung und Sicherheit
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Anfrage
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FAQ
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