Molekulare Biologie
Versatile Cell Transfection Reagents – Meeting the Needs of Every Cell Type and Nucleic Acid
Cell transfection is a technique used to introduce exogenous nucleic acids (such as DNA, mRNA, siRNA, miRNA, etc.) into cells, and it plays a criti...
Principles and Troubleshooting of Plasmid Extraction
Plasmid extraction is a fundamental molecular biology technique used to isolate plasmid DNA from bacterial cells. Depending on the required yield a...
Don’t Know How Much cDNA to Use? Still Measuring cDNA Concentration?
Quantitative PCR (qPCR) is a widely used technique in molecular biology, often perceived as straightforward. However, successful qPCR experiments h...
Common Issues and Troubleshooting in RNA Extraction Using TRIzol
TRIzol reagent mainly contains phenol and guanidine isothiocyanate. These components rapidly lyse cells while inhibiting the activity of RNases rel...
Are You Choosing the Right Method for Nucleic Acid Quantification?
Nucleic acid extraction is a routine procedure in most molecular biology labs. The extracted DNA or RNA is often used in downstream applications su...
Troubleshooting Nucleic Acid Gel Electrophoresis—Solved in One Go!
Introduction
Agarose gel electrophoresis is likely one of the first experiments you performed in the lab. Today, let's take a deep dive into this...
GMP-Grade Recombinant Human bFGF/FGF2 — A Key Factor in Stem Cell Culture
01 Overview
Basic Fibroblast Growth Factor (bFGF), also known as Fibroblast Growth Factor-2 (FGF-2), is a member of the FGF family, which include...
Why Does Your Melt Curve Look So Strange?
Troubleshooting Abnormal Melt Curves in qPCR
In qPCR experiments, melt curves are essential for ensuring product specificity and data accuracy. Abn...
Why Do Amplification Curves Show Spikes?
Perfect amplification curves follow a predictable pattern, but anomalies can appear in all sorts of strange shapes. One common issue, especially wi...
Kostenlose Anleitung: Top 5 PCR -Optimierungs -Tipps
PCR (Polymerase-Kettenreaktion) ist eine grundlegende Technik in der Molekularbiologie und wird häufig für Genklonierung, diagnostische Tests und F...
PCR, Nukleinsäurelektrophorese, Handbuch für Labortechniken
Kapitel 1: PCR-Technologie 1.1 Prinzipien der PCR PCR (Polymerase Chain Reaction) oder Polymerase-Kettenreaktion bezeichnet die DNA-Polym...
Hieff-Klon Universal II One Street Cloning Kit--1–7 Fragment-ein-Schritt-Kloning
Stört Sie immer noch die geringe Effizienz der homologen Rekombination von Multifragmenten oder superlangen Fragmenten und die geringe Anzahl von K...