Yeasen and Molefuture Introduce Advanced T4 DNA Ligase to Overcome Molecular Biology Challenges
Yeasen and Molefuture have jointly developed a cutting-edge T4 DNA ligase using their ZymeEditor platform, addressing key issues in molecular biology and DNA library construction for next-generation sequencing (NGS). Traditional T4 DNA ligase faces challenges such as poor stability, high adapter dimer formation, and high DNA fragment self-ligation. This novel enzyme aims to overcome these limitations.
T4 DNA ligase, an ATP-dependent enzyme, features a compact-helical DNA-binding domain (DBD), a nucleotidyl-transferase (NTase) domain, and an OB-fold domain. By analyzing protein structure, researchers identified key residues within 5 to 20Ć of the DNA substrate. Using advanced computational tools, in silico screening, and structure-function analysis, they designed mutants through consensus sequence alignment, incorporating structural-functional insights.
In addition to rational design, the team employed the MTPS method for directed evolution, screening over 10,000 mutants for stability, activity, and residual activity. Promising candidates underwent rigorous purification and testing. Detailed characterization followed, evaluating activity, stability, adapter self-ligation, DNA fragment self-ligation, and DNA yield.
The researchers combined DNA rational design and directed mutation on G1 mutants to develop exceptional variants suited for diverse applications. This comprehensive approach has resulted in a highly efficient and reliable T4 DNA ligase, poised to enhance molecular biology research and DNA library construction in NGS.
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Ordering information
Name | Cat# | Notes | |
Enzyme | Hieffā¢ Versatile T4 DNA Ligase (600 U/ĀµL) | 12996ES | Thermotolerant and High Fidelity for Tumor gene detection |
DNA | Hieff NGSĀ® DNA Library Prep Kit | 13577ES | Tumor/ Mechanic method |
Hieff NGSĀ® OnePot Pro DNA Library Prep Kit V2 | 12194ES | Tumor/ Enzymetic method | |
Hieff NGSĀ® OnePotĀ II DNA Library Prep Kit for Illumina | 13490ES | Pathgen/ Enzymetic/ regular time (140min) | |
Hieff NGSĀ® OnePot Flash DNA Library Prep Kit | 12316ES | Pathgen/ Enzymetic/ UltrafastĀ (100min) | |
Hieff NGSĀ® DNA&RNA Library Co-Prep Kit V2 | 12305ES | Pathgen/ Enzymetic/ DNA & RNA Co-Prep | |
RNA | Hieff NGSĀ® Ultima Dual-mode mRNA Library Prep KitĀ | 12308ES | Without oligo dT magnetic beads, 11 tubes |
Hieff NGSĀ® Ultima Dual-mode mRNA Library Prep KitĀ Ā | 12309ES | oligo dT magnetic beads plus, 14 tubes | |
Hieff NGSĀ® Ultima Dual-mode RNA Library Prep KitĀ | 12310ES | Premixed version, 5 tubes | |
Hieff NGS Ā® EvoMax RNA Library Prep Kitļ¼Premixed versionļ¼(actinomycin DĀ Free) | 12340ES | Premixed version,Ā (Actinomycin DĀ Free) | |
Hieff NGSĀ® MaxUp rRNA Depletion Kit (Plant)Ā | 12254ES | Plant | |
Hieff NGSĀ® MaxUp Human rRNA Depletion Kit (rRNA & ITS/ETS) | 12257ES | Human |
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About Molefuture Biotechnology
Molefuture Biotechnology is a subsidiary of YeasenĀ Biotechnology (Shanghai) Co., Ltd., specializing in providing customized enzyme modification solutions driven by enzyme evolution technology. Leveraging the resources of YeasenĀ Biotechnology, Molefuture operates on six major technological platforms: the ZymeEditor innovative enzyme evolution platform, a multi-host high-efficiency expression platform, fermentation process development platform, purification process development platform, ultra-clean enzyme production platform, and enzyme analysis and quality control platform. With these six technological platforms, Molefuture can offer a complete set of customized solutions including enzyme-directed evolution, new enzyme development, enzyme process development, and GMP-level scale-up production to meet the application demands of enzymes in areas such as in vitro diagnostics, biomedicine, synthetic biology, medical aesthetics, pharmaceutical intermediates, et. al.