Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides that regulate gene expression and chromatin organization. With the development of RNA-Seq technologies, lncRNA research has expanded in cancer biology, epigenetics, and precision medicine.
Because many lncRNAs are low-abundance transcripts, library preparation quality is critical for accurate detection.
Key Applications
|
Application |
Description |
|
lncRNA discovery |
Identify novel regulatory RNAs |
|
Gene regulation studies |
Investigate RNA-mediated regulation |
|
Cancer research |
Identify disease-associated lncRNAs |
|
Biomarker identification |
Discover diagnostic markers |
Industry Challenges
|
Challenge |
Impact |
|
Low expression levels |
Difficult detection |
|
rRNA background |
Reduces sequencing efficiency |
|
Complex transcript structures |
Complicates annotation |
Yeasen lncRNA Library Prep Workflow
Yeasen provides optimized rRNA depletion-based library preparation workflows designed for high efficiency across diverse sample types, including human, mouse, and rat samples, plant tissues, and blood samples requiring globin depletion.
Library Preparation Tips
|
Factor |
Recommendation |
|
RNA quality |
High-quality RNA improves detection |
|
Strand specificity |
Important for lncRNA annotation |
|
Sequencing depth |
50–100M reads recommended |
FAQ
Q1. What is total RNA sequencing?
Total RNA-seq captures all RNA species in a sample after rRNA removal, including mRNA, lncRNA, circRNA, and other non-coding RNAs.
Q2. When should I choose total RNA-seq instead of mRNA-seq?
Total RNA-seq is recommended when studying global transcriptome profiles, RNA processing, or when investigating coding and non-coding RNA simultaneously.
Q3. Does total RNA-seq require more sequencing depth?
Yes. Because total RNA-seq includes a broader range of RNA species, it often requires higher sequencing depth compared to mRNA-seq.
Q4. What samples are suitable for total RNA-seq?
Total RNA-seq works well for degraded samples, FFPE tissues, and complex transcriptome studies, especially when rRNA depletion strategies are used.
Related Product
|
Category |
Name |
Cat. No. |
Size |
|
|
RNA Lib Prep |
Dual-mode(Strand specific & Non Strand specific) |
12308ES24/96 |
24 T/96 T |
|
|
Premix version |
12340ES24/96 |
|||
|
12341ES24/96 |
||||
|
mRNA isolation |
Eukaryotic mRNA |
12629ES24/96 |
24 T/96 T |
|
|
rRNA depletion |
Human/Mouse/Rat |
Hieff NGS™ MaxUp Human/Mouse/Rat rRNA Depletion Kit(rRNA ITS/ETS) |
12257ES24/96 |
|
|
Hieff NGS™ MaxUp Human/Mouse/Rat rRNA Depletion Kit(rRNA ITS/ETS) 2.0 |
12726ES24/96 |
|||
|
Plant |
12254ES24/96 |
|||
|
Beads |
- |
12602ES03/08/56 |
1/5/60 mL |
|