Is there the lab EB?refuse EB! With the non-toxic nucleic acid dye YeaRed/YeaGreen!
Ethidium bromide (EB) is the earliest and most mature nucleic acid dye, and because of its cheap price and high sensitivity, it was widely used by experimenters. But its molecular weight is small, can penetrate cell membrane very easily, belong to mutagen, have high carcinogenicity, the potential harm to human body is very big. The experimenter needs to be careful, careful and careful. In addition, the separate EB area occupies a large piece of space in the laboratory. The disposal of waste glue, waste liquid is time-consuming, laborious and expensive, and accidentally encounter the EB area may not see the light of day, which is really people love and hate.
With the development of technology, people pay more attention to health and environmental safety, and non-toxic nucleic acid dyes come into being. So, are the declared non-toxic nucleic acid dyes really non-toxic? What are the advantages of being non-toxic?
With these questions in mind, YEASEN will introduce two safe and non-toxic nucleic acid dyes that can replace EB- -YeaRed and YeaGreen.
YeaRed（10202ES）
YeaRed is a new non-toxic nucleic acid dye developed by YEASEN, has a unique oily macromolecular structure, can not penetrate the cell membrane into the cell, not volatile sublimation, the human body will not inhale, so as to ensure the safety of the experimenter to the greatest extent. In addition, the YeaRed-nucleic acid dye was subjected to the Mini-Ames test and was negative, that is, the YeaRed-nucleic acid dye was not toxic at the gel staining concentration.(The specific test report can be consulted to our company)
Fig.1 YeaRed spectrogram
YeaRed-Six-majorproduct-features
Excellent Performance
Fig.2 Agarose gel electrophoregram of the nucleic acid dye YeaRed with EB
Samples were 100 bp DNA ladder and 2000 DNA Marker, loaded with 2,3,4, and 5 μL, respectively; electrophoresis condition: 140 V, 40 min.
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XX University
XXX University
YeaGreen（10204ES）
YeaGreen Another new type of non-toxic nucleic acid dye newly developed by YEASEN, can not penetrate the cell membrane into the cell, not volatile sublimation, the human body will not inhale. The Ames test results also indicate that YeaGreen is completely unmutagenic at gel staining concentrations and therefore completely replaces EB.
It is not only non-toxic, but also has the same spectral properties as SYBR Green I (low toxicity), and the impact on the nucleic acid mobility is much less than that of SYBR Green I. It is suitable for observation and glue cutting under blue light and ultraviolet light conditions.
Fig.5 YeaGreen spectroscopy
Excellent performance under blue light conditions
Product Details
Q&A
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Questions |
Answer |
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Usage method |
1. What are the methods of YeaRed / YeaGreen nucleic acid dyes? |
Rubber dye method, bubble dye method can be. |
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2. Does YeaRed / YeaGreen nucleic acid dyes need a special electrophoretic buffer solution? |
Not required, and YeaRed / YeaGreen is compatible with almost all commonly used electrophoretic buffer solutions. |
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3. What observation device is required to use the YeaRed / YeaGreen nucleic acid dye? |
YeaRed Has the same spectral properties as EB and was observed with the same UV gel imager as EB. YeaGreen Has the same spectral properties as SYBR Green I, which can be used for blue light imager or for UV gel imager. |
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4. Can the YeaRed / YeaGreen nucleic acid dye used after the bubble dye method be reused? |
Yes, and it is reusable for about 3 times. |
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5. How to deal with the discarded YeaRed / YeaGreen nucleic acid dyes? |
YeaRed / YeaGreen nucleic acid dyes will naturally decompose in the environment and can be treated as solid waste. |
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Scope of application |
1. What are the applicable sample types for YeaRed / YeaGreen nucleic acid dyes? |
Suitable for electrophoretic staining of various size fragments of dsDNA, ssDNA and RNA. |
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2. Which gel electrophoresis is the YeaRed / YeaGreen nucleic acid dye suitable for? |
In addition to agarose gel electrophoresis, it is also suitable for polyacrylamide gel electrophoresis, but polyacrylamide gel electrophoresis can only use the bubble-dye method. |
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3. What downstream experiments are suitable for the YeaRed / YeaGreen nucleic acid dyes? |
Compatible glue recovery, as well as downstream experiments such as cloning and sequencing. |
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Product features |
1. What is the stability of the YeaRed / YeaGreen nucleic acid dye? |
At room temperature, extremely stable in acid or alkali buffer, strong light resistance. The preparation can also be heated directly heated in the microwave oven. |
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2. What is the sensitivity of the YeaRed / YeaGreen nucleic acid dye? |
YeaRed is a highly sensitive nucleic acid dye with comparable sensitivity to EB. |
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3. What is the binding mechanism of YeaRed / YeaGreen? |
By a combination of electrostatic and charge interactions. |
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4. How are YeaRed / YeaGreen nucleic acid dyes preserved? |
Keep at room temperature, YeaRed for 5 years and YeaGreen for 3 years. |
Published Literature (Partial)
[1] Li X, Zhang Y, Xu L, et al. Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease. Cell Metab. 2022.doi:10.1016/j.cmet.2022.10.002. IF=31.373(10202ES)
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