D-Luciferin is a common substrate for the luciferase enzyme Luciferase and is commonly used throughout biotechnology, especially in in vivo in vivo imaging. The mechanism of action is that luciferin (the substrate) can be oxidized to emit light in the presence of ATP and luciferase. When there is an excess of luciferin, the number of light quanta produced is positively correlated with the concentration of luciferase (see figure below). After transfecting cells with a plasmid carrying the gene encoding luciferase (Luc) and introducing it into research animals such as mice and rats, luciferin is injected into the cells, and changes in light intensity are detected by BLI, allowing real-time monitoring of the state of disease progression or the therapeutic efficacy of a drug, for example. The effect of ATP on this reaction system can also be utilized to indicate energy or vital signs based on changes in bioluminescence intensity.

D-Luciferin is also commonly used in in vitro studies including luciferase and ATP level analysis, reporter gene analysis, high throughput sequencing, and various contamination assays. There are currently three product forms on the market, D-Luciferin (free acid), D-Luciferin sodium salt, and D-Luciferin potassium salt. The main difference between these three products is in their solubility characteristics; D-Luciferin (free acid) is weakly soluble in water and in buffer systems, unless it is soluble in weak bases such as NaOH and KOH solutions. D-Luciferin in the form of sodium and potassium salts dissolves very easily and quickly in water or buffers, is easy to use, and the solvents are non-toxic, making it particularly suitable for in vivo experiments. When formulated as liquids, there is no substantial difference between the three products for most applications.

Features

No radiation, almost harmless to living organisms.

Bioluminescence, no excitation light source.

So sensitive, you can detect it in a few hundred cells.

Good penetration, 3-4cm tissue depth can still be detected.

High signal-to-noise ratio, strong fluorescence signal and good anti-interference.

Applications

In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.

To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.

The localization and distribution of foreign cells in animals were detected.

The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.

Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.

Specifications

Components