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T4 Endonuclease V, also known as T4 PDG (Pyrimidine Dimer Glycosylase), is a bifunctional DNA glycosylase that combines the activities of DNA N-glycosylase and AP lyase. This enzyme is capable of recognizing and binding to cis-syn cyclobutane pyrimidine dimers, which are induced by ultraviolet radiation. It then cleaves the glycosidic bond at the 5' end of the pyrimidine dimers, releasing them and leaving an AP site. Subsequently, it utilizes its endonuclease activity to cleave the phosphodiester bond at the AP site, creating a one-nucleotide gap in the DNA strand.
Applications
DNA damage studies
Single cell gel electrophoresis (comet assay)
Specifications
|
Source |
T4 Phage |
|
Molecular Weight |
16.1 KD |
|
Concentration |
1 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 µL in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers |
Components
|
Components No. |
Name |
14542ES76 |
14542ES80 |
|
14542-A |
T4 Endonuclease V(T4 PDG)(1 U/μL) |
500 μL |
1 mL |
|
14542-B |
10 x T4 Endonuclease V(T4 PDG) Buffer |
1 mL |
2 mL |
Storage
FAQ
Citations
Applications
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