Frequently Bought Together
This product is an inorganic pyrophosphatase derived from recombinant expression in Escherichia coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions. This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.
Feature
Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases
Increasing RNA yield in transcription reaction; enhancing DNA replication
Application
DNA sequencing reaction
PCR and single-base extension reaction
In vitro transcription processes
Specification
| Source | Recombinant E.coli with Pyrophosphatase gene from yeast |
| Optimum Temperature | 25℃ |
| Storage Buffer | 50 mM Tris-HCl pH 7.9，100 mM NaCl，10 mM DTT，1 mM EDTA，0.1% （v/v）TritonX-100，50%（v/v）glycerin |
| Unit Definition | One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi. (The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min) |
Components
| Components No. | Name | 10620ES10 (10 U) |
10620ES60 (100 U) |
10620ES80 (1,000 U) |
10620ES99 (40 KU) |
| 10620 | Pyrophosphatase, Inorganic GMP-grade (1 U/μL) | 10 μL | 100 μL | 1 mL | 40 mL |
Figures
Figures 1. The relationship between RNA yield and IPPA concentration in vitro transcription
Storage
FAQ
Citations
Documents
Applications
You may also like
Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.