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dUTP, or 2 '-deoxyuridine 5'-triphosphate, is commonly used in PCR, qPCR, RT-PCR and RT-qPCR to replace dTTP, so that the product of amplification contains uracil. When the UDG is added to the amplification reaction system, the uracil of any contaminated amplification products can be removed, so as to avoid the false positive.
This product is in the form of solution sodium salt, prepared with ultrapure water, and adjusted to pH value of 7.0 with high-purity NaOH solution, with a concentration of 100 mmol/L.
The purity of dUTP was ≥99% (HPLC), and there was no DNase and RNase pollution. This product is ready to use and can be directly used in PCR and other conventional molecular biological reactions.
Features
Greater than 99% purity—confirmed by HPLC
Free of human and E. coli DNA
Highly stable
Applications
Long range PCR (40 kb)
cDNA synthesis and RT-PCR
Real-time PCR
Standard PCR
High fidelity PCR
Specifications
| Concentration | 100 mM |
| CAS NO. | 102814-08-4 |
| Form | Liquid |
| Purity | >99% |
| Label or Dye | Unlabeled |
| For Use With (Application) | PCR Amplification |
Components
| Components No. | Name | 10128ES74 | 10128ES80 | 10128ES96 | 10128ES97 |
| 10128 | dUTP, 100 mmol/L Solution | 400 μL | 1 mL | 25 mL | 400 mL |
Storage
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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