You reach the last row of a 96-well plate and stop. Did that well get its sample?
You check the plate again, but clear solutions offer few clues. When a result looks unusual later, the same question comes back: did I miss an addition?
That uncertainty is familiar to anyone who runs RT-qPCR. The ColorGPS pair makes sample addition visible without changing your routine. Instead of relying on memory alone, you get a color cue built into the reagents at each step.
Red Plus Blue Makes Purple
YEASEN ColorGPS pairs a red reverse transcription mix(Cat. No. 11157ES), with a blue universal qPCR mix(Cat. No. 11184ES/11186ES). Add the red cDNA to the blue qPCR mixture, and the reaction turns purple.
The pair brings visual tracking from reverse transcription through qPCR, together with convenient handling, broad instrument compatibility, and strong reaction performance. It helps you follow sample addition throughout setup and supports reliable quantitative results.
Red cDNA generated with 11157ES + blue qPCR mix = a purple reaction after cDNA addition.
Start with Red ColorGPS Reverse Transcription
Hifair ColorGPS All-in-One RT-gDNA Digestion SuperMix for qPCR (Cat. No. 11157ES) offers a visible, one-tube, one-step reverse transcription workflow. It is designed to address common frustrations: missed additions, complicated handling, genomic DNA interference, and low reverse transcription efficiency.
One Premix Completes gDNA Digestion and Reverse Transcription in 15 Minutes
All components needed for gDNA digestion and reverse transcription are combined in a single premix. Add your RNA template and water to complete both reactions in 15 minutes. There is no need for repeated tube opening or separate reaction stages, reducing handling and the risk of nucleic acid aerosol contamination. The simpler setup also makes the workflow easier for new users to learn.
A single premix converts RNA into red cDNA while combining gDNA digestion and reverse transcription in a 15-minute workflow.
≥99.8 Percent gDNA Digestion
YEASEN tested 11157ES with 200 ng and 400 ng of human 293T cell genomic DNA, comparing digested and undigested samples. Digestion exceeded 99.8% at both input levels, including effective removal at 400 ng. These results support reducing genomic DNA interference in downstream qPCR under the conditions tested.
Amplification and melt curves for digested and undigested 200 ng and 400 ng gDNA samples, with a no-template control (NTC).
Efficient Reverse Transcription Across RNA Sources
The reverse transcription comparison used human, Arabidopsis, and mouse RNA. Downstream qPCR compared cDNA generated with 11157ES against a mainstream alternative, Supplier N*. These results support efficient reverse transcription, with a particularly clear advantage for the mouse RNA samples.
The plotted values show lower Ct values with 11157ES for seven of the nine targets, including all three mouse targets. Human Gene 2 and Arabidopsis Gene 5 show slightly higher Ct values with 11157ES. The figure shows how the comparison varies by target.
Downstream qPCR Ct values after reverse transcription with 11157ES or Supplier N*. Human: 18.0/18.6, 20.4/20.3, 22.0/22.4; Arabidopsis: 19.4/19.6, 22.2/22.0, 24.9/25.6; mouse: 12.6/13.1, 21.4/22.1, 23.9/25.3. Each pair lists 11157ES first.
Continue with Blue Universal qPCR Mix
Hieff UNICON Universal qPCR SYBR Green Master Mix (Cat. No. 11186ES/11184ES) combines broad instrument compatibility, visible sample tracking, and efficient amplification. It is especially useful for batch sample preparation on 96-well and 384-well plates.
Compatibility Across Different qPCR Instruments
YEASEN compared 11184ES with Supplier N* on the Bio-Rad CFX96, ABI QuantStudio 5, and ABI StepOne. These instruments represent no-ROX, low-ROX, and high-ROX settings, respectively. In the displayed experiments, 11184ES produced lower Ct values and stronger fluorescence signals than the comparator, supporting compatibility and amplification performance across the tested platforms.
Amplification and melt-curve comparisons on Bio-Rad CFX96 (no ROX), ABI QuantStudio 5 (low ROX), and ABI StepOne (high ROX). Original experimental traces are retained.
Related products
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Product category |
Product name |
Cat.NO. |
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Reverse transcription |
Hifair ColorGPS All-in-One RT-gDNA Digestion SuperMix |
|
|
qPCR Mix |
Hieff UNICON™ Universal Blue qPCR Master Mix(SYBR) |
|
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Hieff UNICON™ Universal ColorGPS qPCR SYBR Green Master Mix |
11186ES |