Validated, high-efficiency transfection protocols for a wide range of tumor cell lines—helping you achieve optimal results with minimal optimization, right from the first attempt. We provide dedicated experimental protocols for commonly used tumor cells, covering key aspects including cell growth, culture conditions, passaging procedures, and critical steps for highly efficient transfection.

These optimized protocols offer detailed, step-by-step guidance to achieve high transfection efficiency under low cytotoxicity conditions. Validated by numerous academic institutions and research centers worldwide, our solutions are designed to meet real-world experimental needs and enable faster, more reliable results.

Optimized Transfection Protocols for Tumor Cells

Cell Name

Cell name abbreviation

Nucleic Acid Type

Human T-lymphoblastic leukemia cells

Jurkat

DNA

Human cervical cancer cells

Hela

DNA

Mouse colon cancer cells

MC38

DNA

Human gastric adenocarcinoma cells

AGS

DNA

Human hepatocellular carcinoma cells

HepG2

DNA

Human colon cancer cells

HCT-116

siRNA

Human gastric cancer cells

HGC-27

siRNA

Human bladder transitional cell carcinoma cells

T24

DNA

Human non-small cell lung carcinoma cells

A549

DNA

Mouse Lewis lung carcinoma cells

LLC

siRNA

Rat adrenal pheochromocytoma cells

PC-12

DNA

Human Gastric Carcinoma Cells

SNU-719

siRNA

Human Monocytic Leukemia Cells

THP-1

siRNA

Human Osteosarcoma Cells

U2OS

DNA

Human glioblastoma cells

U251

DNA

Human Colorectal Carcinoma Cells

HCT-116

DNA

Human T-Lymphocyte Leukemia Cells

Jurkat

DNA

Human Breast Cancer Cells

MCF-7

siRNA

Human Pancreatic Cancer Cells

MIA PaCa2

DNA

Human Lung Squamous Carcinoma Cells

NCI-H520

siRNA

Human Nasopharyngeal Carcinoma Cells

S18

DNA

Human Monocytic Leukemia Cells

THP-1

DNA

Human Glioblastoma Cells

U251

siRNA

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