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Cell Transfection | Transfection of Primary Mouse Hepatocytes(PMHs)-DNA
Reagents List
Product Name
Cat.NO.
Hieff Trans™ Booster DNA/RNA Transfection Reagent
40801ES
PMHs-DNA Transfection Protocol
Cell Cu...
Cell Transfection | Transfection of Primary Human Skin Fibroblasts(HSFs)-DNA
Reagents List
Product Name
Cat.NO.
Hieff Trans™ Booster DNA/RNA Transfection Reagent
40801ES
Primary Human Dermal Fibroblasts Trans...
Cell Transfection | Transfection of Primary Mouse Skin Fibroblasts(MSFs)-DNA
Reagents List
Product Name
Cat.NO.
Hieff Trans™ Booster DNA/RNA Transfection Reagent
40801ES
Mouse Skin Fibroblasts Transfection...
Cell Transfection | Isolation, Culture, and Transfection of Bone Marrow-Derived Macrophages (BMDMs)
Reagents List
Product Name
Cat.NO.
Hieff Trans™ Booster DNA/RNA Transfection Reagent
40801ES
Hieff™ Optimal-MEM Reduced Serum Mediu...
Cell Transfection | Experimental Protocol for Primary Extraction, Culture, and Transfection of Bone Marrow-Derived Macrophages (BMDM)
I. Cell Extraction and Culture
1. Preparation:
1) Mouse Handling: Sacrifice mice by cervical dislocation, then immerse in 75% ethanol for approxi...
Troubleshooting Transfection: Common Problems and Solutions
I. Principles of Transfection
Q1: What is the difference between transfection and transduction?
Transfection: Introduction of naked or carrier-c...
Protocol for Transfection of mRNA into Cells
1. Experimental Materials
HEK 293 cell: Cell confluency should reach 70%–90%.
mRNA: EGFP mRNA, 1000 bp (Concentration ≥ 100 ng/μL)
Hieff Tr...
Protocol for Transfection of siRNA into Cells
1. Experimental Materials
HEK293 cells: Cell confluency should reach 30%–50% at the time of transfection.
siRNA: Target-specific or control s...
Protocol for Transfection of Primary Bone Marrow-Derived Macrophages (BMDMs)
1. Experimental Materials
BMDM Cells
Target plasmid DNA (Concentration ≥ 1000 ng/μL)
Hieff TransTM Booster DNA&RNA Transfection Reagent...
Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection Reagent (Get Free Trial at the Bottom of the Page)
High toxicity, low efficiency, and poor compatibility have long been the three major challenges in cell transfection.Traditional transfey. For pri...