UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.  It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.

UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.

Features

• Wide range of applications: degrade all forms of DNA and RNA 
• High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10⁶ U/mg
• Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
• Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
• Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
• GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture

Application

• Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
• Reduction of viscosity caused by nucleic acids
• Sample preparation in electrophoresis and chromatography
• Prevention of cell clumping

Specification

Components

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.

Figures

• Quality

• UltraNuclease Enzymatic Activity Under Different Conditions

Temperature & pH

Figure 1. Effects of temperature and pH on enzyme activity.

(A) Enzyme activity increases with temperature, with the optimum at 37 °C.

(B) The enzyme is active within pH 4–10, with pH 8.0 as the optimum.

Salt ion concentration

Figure 2. Effects of salt ion concentration on enzyme activity.

(A). Na⁺/K⁺ inhibit enzyme activity; activity is completely lost above 300 mM, while good activity is retained below 20 mM.

(B). Maximum activity is observed with 5 mM Mg²⁺; in the absence of Mg²⁺, 1–2 mM Mn²⁺ can support activity.

(C). Increasing PO₄³⁻ concentration significantly inhibits enzyme activity.

Surfactants

Figure 3. Effects of surfactants on enzyme activity.

(A) Triton® X-100 and (B) sodium deoxycholate reduce enzyme activity and should be maintained at low concentrations.

Protein denaturants

Figure 4. Effect of protein denaturants on enzyme activity.

SDS and urea reduce enzyme activity and should be maintained at low concentrations.

Other commonly used reagents

Figure 5. Effects of different inhibitors on UltraNuclease activity.

(A). Enzyme activity is strongly influenced by ammonium sulfate concentration.

(B). PMSF shows little effect on enzyme activity.

(C). At low EDTA concentrations (≤0.5 mM), enzyme activity is largely unaffected, whereas at 5 mM EDTA, activity is completely inhibited.

• Robust Performance

Figure 6. Single-factor test comparison with Supplier M*.

No significant difference was observed between Yeasen UltraNuclease and supplier M*.

Strong stability

Figure 7. Stability of UltraNuclease.

(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.

(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.

• Reaction conditions recommend

Table 1. The optimal reaction conditions for UCF.ME™ UltraNuclease

Documents:

20157_Manual_HB221121_EN.pdf

Related Blog:

UltraNuclease (Benzonase Nuclease) – The total Solution for Nucleic Acid Residue removing in Biologics

YEASEN UCF. ME ® UltraNuclease — Your BEST choice for degrading nucleic acids