Hieff Unicon™ Pro Universal TaqMan qPCR Mix(UDG Plus)-16710ES

SKU: 16710ES03

Size: 100 T
Price:
Sale price$50.00

Shipping calculated at checkout

Stock:
In stock
Bulk Quote Inquiry

Description

Hieff Unicon™ Pro Universal TaqMan qPCR Master Mix (UDG plus) is a fluorescent quantitative qPCR reagent that uses the company's next-generation antibody method to heat start Taq enzyme. The 2x TaqMan qPCR Mix pre mixed reagent includes reaction buffer and enzyme mix, already containing Mg2+  and dNTP, and adding factors that effectively inhibit non-specific PCR amplification and improve the efficiency of multiple qPCR reactions, which can ensure primer efficiency while conducting up to four reactions. This product contains UDG enzyme, which can effectively prevent the risk of aerosol pollution.

Components

Components No.

Name

16710ES60

100T

16710ES80

1,000T

16710ES92

10,000T

16710ES94

20,000T

16710

2×TaqMan qPCR Mix

1.25 mL

12.5 mL

125 mL

250 mL


Specifications

Hot Start

Built-in hot start

Detection method

Primer-probe detection

PCR method

qPCR

Polymerase

Taq DNA polymerase

Type of sample

DNA

 

 

 

 

 

 

Application equipment

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus

™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384,  iCycler iQ,  iQ5,  MyiQ,  MiniOpticon,  Opticon,  Opticon  2,

Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.

Instructions

1.Reaction System

Components

Volume (μL)

Final Concentration

2×TaqMan qPCR mix

12.5

Primer mix (10  μmol/L)

x

0.1-0.5  μmol/L

Probe mix (10  μmol/L)

x

50-250 nmol/L

Rox reference dye

0.5

Template DNA/cDNA

1-10

-

ddH2O

up to 25

-

*The primer concentration can be adjusted appropriately according to the experimental requirements.

2.  Reaction program

 

Cycle step

Temp.

Time

Cycles

UDG treatment

37℃

5 min 

1

Initial denaturation

95℃

5 min

1

Denaturation

95℃

15 s

 

45

Annealing/Extension

60℃

30 s

*The annealing / extension temperature can be adjusted appropriately according to the experimental requirements.

Notes

Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!

Inquiry

You may also like