Description
Restriction endonuclease Not I is derived from the recombinant expression of the NotIR gene in Nocardia otitidis-caviarum. This enzyme has the activity to recognize the cleavage site 5'-GC↓GGCCGC-3', specifically cleaving double-stranded DNA to produce sticky ends. It is commonly used for single and double digestions. This product belongs to the fast-digest enzyme series and can rapidly complete the digestion of substrates such as plasmid DNA, PCR products, and genomic DNA within 15 minutes.
Application
Molecular Cloning
Plasmid Linearization
Specifications
|
Recognition site |
5'-GC↓GGCCGC-3' 3'-CGCCGG↑CG-5' |
|
Concentration |
10 U/µL |
|
Inactivation Conditions |
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA within 1 hour at 37°C in a total reaction volume of 50 µL |
|
Reaction conditions |
React at 37℃ for 15-30 min |
|
Inactivation conditions |
React at 65℃ for 20 min |
Components
|
Component No. |
Name |
15208ES76 |
15208ES86 |
|
15208-A |
Not I (10 U/μL) |
50 μL |
250 μL |
|
15208-B |
10×Not I Reaction Buffer |
1.25 mL |
1.25 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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