Description
Hieff NGS™ RNA Lib Prep 384 CDI Primer for Illumina is a dedicated linker primer kit for RNA library construction of the Illumina high-throughput sequencing platform, divided into 2 Sets, each Set contains the PE adapter used in the second-generation sequencing library and 8 of i5 Index Primer and 12 of i7 Index Primer. A total of 16 of i5 Index Primers and 24 of i7 Index Primers are available in 2 Sets which make 384 different combinations of double-ended index-labeled libraries can be constructed. All reagents provided in the kit undergo rigorous quality control and functional verification to maximize library construction stability and repeatability.
Components
|
No. |
Name |
Volume |
|
12414ES02 |
PE Adapter |
320 μL |
|
RP 501-508 |
60 μL each |
|
|
RP 701-712 |
40 μL each |
|
|
12415ES02 |
PE Adapter |
320 μL |
|
RP 509-516 |
60 μL each |
|
|
RP 713-724 |
40 μL each |
Shipping and Storage
All the components are shipped with dry ice and can be stored at -15℃~ -25℃ for 18 months.
Note:
2) The PE Adapter provided with this kit is a general-purpose short adapter that requires PCR amplification to obtain a complete library. Index Primer provides Barcode sequence tags for distinguishing samples during high-throughput sequencing.
3) Each set of this kit contains 8 of i5 Index Primers (RP 5xx) and 12 of i7 Index Primers (RP 7xx), which can be combined with each other to construct 96 different combinations of double-ended Index labeled libraries. 2 Sets offer a total of 16 of i5 Index Primers and 24 of i7 Index Primers. You can use the i5 Index Primer (or i7 Index Primer) from Cat#12414 and the i7 Index Primer (or i5 Index Primer) in Cat#12415 to build up to 384 different combinations of double-ended Index labeled libraries.
4) Do not heat the adapter, let it dissolve slowly at room temperature, and the laboratory temperature is best set to 20-25 °C. The adapters are need to avoid repeated freeze-thaw, and it is recommended to store it in aliquots and store it at 4°C for a short time.
5) The sequencing library structure constructed using the Hieff NGS® RNA 384 CDI Primer for Illumina™ kit is as follows:
6) For your safety and health, please wear a lab coat and wear disposable gloves.
7) This product is for scientific research purposes only!
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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