Dual Glo Luciferase Reporter Gene Assay Kit _ 11405ES

Firefly luciferase is a protein with a molecular weight of about 61 kDa, which can catalyze the oxidation of luciferin to oxyluciferin in the presence of ATP, magnesium ions and oxygen. It emits bioluminescence with a wavelength of around 560 nm. Renilla luciferase is a protein with a molecular weight of about 36 kDa. In the presence of oxygen, it can catalyze the oxidation of coelenterazine to coelenteramide. During the oxidation process, it emits a wavelength of 480 nm. about bioluminescence. Both bioluminescence can be measured by chemiluminescence. The detection principle is shown in the figure1: 

Figure 1: Schematic diagram of firefly and Renilla luciferase assays

Dual Glo Luciferase Reporter Gene Assay Kit is a glow-type dual luciferase reporter gene detection kit with high sensitivity and stable luminescence signal. This kit contains high-purity firefly luciferin and coelenterazine. In the same sample, firefly luciferase is used as a substrate to detect firefly luciferase, and then the fluorescence signal of firefly luciferase is quenched, and at the same time coelenterazine is used as a substrate to detect Renilla luciferase to achieve dual luciferase reporter gene detection. Renilla luciferase is used as an internal reference, which eliminates the influence caused by the number of cells between the wells and the transfection efficiency, which makes the detection results more accurate.

Compared with the flash-type Dual Luciferase Reporter Gene Assay Kit (Cat#11402), this product has the following advantages: 1) The luminescence signal is more stable, providing greater flexibility for experimental design. 2) It is more in line with high-throughput detection, does not need to rely on automatic samplers, and does not need to discard the culture medium, centrifugation and other steps, which greatly simplifies the experimental process. 3) No obvious irritating odor. This kit can be used in a variety of common cell culture media: RPMI 1640, DMEM, MEM-α, F12, DMEM/F12, etc. Its half-life is about 2h (22°C), which meets the needs of most high-throughput experiments.

Features

Signal stability: Long half-life (up to 2 hours), highly suitable for high-throughput detection, no more worries about the quenching of fluorescence signals.

Easy operation: Just add samples and read values, the experiment is completed, freeing up more time to publish papers.

High accuracy: I have the substrate for Renilla luciferase—coelenterazine, which can correct errors in cell number and transfection efficiency between wells.

Good compatibility: Can be used with various commonly used cell culture media, such as RPMI 1640, DMEM, MEM-α, F12, DMEM/F12, etc.

Non-irritating odor: Returning you a fresh and pleasant laboratory environment.

 Verification of miRNA interactions with target gene 3'UTRs

Verification of lncRNA-miRNA interactions

Verification of transcription factor regulation in the promoter region

Signaling pathway analysis

SNP activity analysis

Specifications

Components

Shipping and Storage

The Dual Luciferase Reporter Gene Assay Kit products should be stored at -15℃ ~ -25℃ for 1 year.

Figures

Figure1. YEASEN (Cat#: 11405) has a fluorescence intensity comparable to that of Brand P.

Figure2. YEASEN (Cat#: 11405) detection reagents interact with luciferase to achieve optimal luminescence intensity. After the addition of Renilla luciferase substrate, the quenching effect on firefly luciferase luminescence is comparable to that of the imported P brand.

Documents:

Safety Data Sheet

11405 MSDS HB220209 EN.PDF

Manuals

11405 Manual HB241230 EN.pdf