Hieff NGS^TM MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS)  utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.

Features

• Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
• Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
• High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
• Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.

Components

Applications

• Gene Expression Research
• Alternative splicing analysis
• Detection and discovery of LncRNA(Non coding RNA)
• dentification of selective polyadenylation sites
• Fusion gene detection

Storage

This product should be stored at -25~-15℃ for 12 months.