Alternative Names

CD47,OA3,CD47 molecule,CD47 glycoprotein

Species

Human

Expression Sequence

Gln19-Pro139

Accession

Q08722-1

Tag

No Tag

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human CD47, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. For initial use, aliquot the protein to avoid repeated freeze-thaw cycles.