Synonyms

TNFCR, TNFR3, TNFRSF3, LTBR, CD18, TNFR2-RP, TNFR-RP, LT-BETA-R, TNF-R-III, D12S370, LT-β-R

Species

Human

Expression Region

Gln31-Met227

Accession

P36941-1

Tag

C-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based onSDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin Level

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Bioactivity

【ELISA Data】Immobilized Human LIGHT, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA . Immobilized Human LTBR, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-LTBR Antibody, hFc Tag with the EC50 20.7 ng/ml determined by ELISA.

Reconstitution

Before opening, briefly centrifuge the vial.
Reconstitute the lyophilized protein using sterile water to a concentration greater than 100 μg/mL.
It is recommended to aliquot the reconstituted protein to avoid repeated freeze–thaw cycles.