Frequently Bought Together
Mouse Bone Marrow-Derived Macrophages (BMDMs) are a primary macrophage model obtained through in vitro induced differentiation. They are generated by extracting bone marrow cells from the femurs and tibias of mice and culturing them for approximately 7 days in the presence of specific cytokines, allowing hematopoietic progenitor cells in the bone marrow to proliferate and differentiate into mature, functional macrophages. Due to their ease of access, ability to be expanded in large quantities, consistent genetic background (allowing the use of genetically modified mice), and their capacity to mimic key characteristics of in vivo macrophages, BMDMs have become one of the most important and widely used primary cell models in fields such as immunology, inflammatory diseases, infection immunity, and oncology.
This kit is used to differentiate mouse bone marrow cells into macrophages. This kit contains four reagents: recombinant mouse M-CSF, recombinant mouse IL-4, recombinant mouse IL-13, and recombinant mouse IFN-γ. M-CSF is the essential and uniquely critical cytokine that drives bone marrow precursor cells to differentiate, proliferate, and survive along the macrophage lineage; a concentration range of 10 - 50 ng/mL is typically used. This concentration is sufficient to support cell proliferation and differentiation without over-activating the cells. Macrophages are classified into two main types: M1-type macrophages and M2-type macrophages. LPS/IFN-γ can stimulate cells to polarize towards the M1 macrophage phenotype. IL-4 and IL-13 act synergistically to drive cells to polarize towards the M2 phenotype.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Mouse Macrophage BMDM Cytokine Set |
92643ES10 |
10 μg |
|
92643ES50 |
50 μg |
Components
|
Component Cat No. |
Component Name |
92643ES10 |
92643ES50 |
Quantity |
|
92643ES-A |
Mouse M-CSF Protein |
10 μg |
50 μg(5×10 μg) |
1 |
|
92643ES-B |
Mouse IL-4 Protein |
10 μg(2×5 μg) |
50 μg |
1 |
|
92643ES-C |
Mouse IL-13 Protein |
10 μg |
50 μg |
1 |
|
92643ES-D |
Mouse IFN-γ Protein |
50 μg |
100 μg |
1 |
|
92643ES-E |
Lipopolysaccharide (LPS) |
5 mg |
5 mg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
Working Concentration (For Reference Only) |
|
91114ES |
Mouse M-CSF Protein |
E. coli |
Lyophilized powder |
20 ng/mL |
|
90144ES |
Mouse IL-4 Protein |
E. coli |
Lyophilized powder |
40 ng/mL |
|
90151ES |
Mouse IL-13 Protein |
E. coli |
Lyophilized powder |
40 ng/mL |
|
91212ES |
Mouse IFN-γ Protein |
E. coli |
Lyophilized powder |
50 ng/mL |
|
60747ES |
Lipopolysaccharide (LPS) |
E. coli |
Lyophilized powder |
100 ng/mL |
The above reagent usage concentrations are for reference only. The concentrations need to be optimized based on specific experimental conditions, purposes, and actual induction effects.
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Mouse IL-4 Protein Mouse IL-13 Protein Mouse IFN-γ Protein |
Centrifuge the vial prior to opening to pellet contents at the bottom. Reconstitute with sterile water to a concentration of 0.1–1.0 mg/mL, and further dilute according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) into the dilution buffer. Aliquot into single-use experimental volumes and store at -80°C; avoid repeated freeze-thaw cycles. |
|
Lipopolysaccharide (LPS) |
Reconstitute with water or cell culture medium to a concentration of 1 mg/mL. |
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
Storage
FAQ
Citations
Documents
당신은 또한 좋아할 수 있습니다
문의
자주 묻는 질문
이 제품은 연구 목적으로만 사용되며 인간이나 동물의 치료 또는 진단용으로 의도되지 않았습니다. 제품과 콘텐츠는
특정 애플리케이션에는 추가적인 제3자 지적 재산권이 필요할 수 있습니다.
예슨은 윤리적 과학에 헌신하며, 우리의 연구가 안전과 윤리적 기준을 보장하는 동시에 중요한 문제를 해결해야 한다고 믿습니다.