Cat.No.

90301ES08 / 90301ES20

Size

5 μg / 20 μg

Name

90301ES08

90301ES20

HiActi™ Recombinant Porcine IL-1 alpha Protein, His Tag

5 μg

20 μg

Synonyms

interleukin 1 alpha, IL1A

Species

Porcine

Expression Sequence

MSATYSFQSNMKYNFMRVINHQCILNDARNQSIIRDPSGQYLMAAVLNNLDEAVKFDMAAYTSNDDSQLPVTLRISETRLFVSAQNEDEPVLLKELPETPKTIKDETSLLFFWEKHGNMDYFKSAAHPKLFIATRQEKLVHMAPGLPSVTDFQILENQS with polyhistidine tag at the C-terminus.

Accession

P18430

Tag

C-His

Expression System

E.coli

Molecular Weight

The protein has a calculated MW of 19.02 kDa. The protein migrates as 17-25 kDa under reducing condition

Purity

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.

Appearance

Lyophilized Powder

Biological activity

Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀ for this effect is <50 pg/mL.

Reconstitution Instructions

Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening.

It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution.

The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier.