설명
The Cell Species Identification Kit (Multiplex PCR Method) is designed for the identification of cell substrate origins, ensuring correct cell line identity and absence of cross-contamination from other cell lines. It enables quality control of raw materials in cell-based biopharmaceuticals.
This kit is based on multiplex PCR amplification of mitochondrial genes from animal cells, including cytochrome b, cytochrome c oxidase I (COX I), and cytochrome c oxidase II (COX II). Species origin is determined by analyzing the amplification products via agarose gel electrophoresis.
Features
- Broad Species Compatibility – Accurately detects 9 different cell species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6) and rhesus monkey (LLC-MK2).
- High Specificity – Reliably identifies contaminating cells even in the presence of primary cells (HEK293, MDCK, Vero, CHO).
- Reliable Results – Based on amplicon band number and size for clear interpretation.
- High Quality & Stable Supply – Ensures consistent performance and reliable availability.
Specifications
|
Cat NO. |
41341ES25 / 41341ES50 |
|
Size |
25 T / 50 T |
Components
|
category |
Components No. |
Name |
41341ES25 |
41341ES50 |
|
Part I |
41341-C |
Cell Extraction Buffer |
1.25 mL × 2 tubes |
1.25 mL × 4 tubes |
|
Part II |
41341-A |
2× Multiplex PCR Buffer |
312.5 μL |
625 μL |
|
41341-B |
Primers Mix |
112.5 μL |
225 μL |
|
|
41341-D |
Proteinase K (20 mg/mL) |
50 μL |
100 μL |
|
|
41341-E |
DL500 DNA Marker |
125 μL |
250 μL |
|
|
41341-F |
DEPC-Treated Water |
1.25 mL |
1.25 mL × 2 tubes |
|
|
41341-G |
Positive Control |
25 μL |
25 μL × 2 tubes |
Storage
1. Part I should be shipped at ambient temperature and stored at room temperature. Shelf life: 2 years.
2. Part II should be shipped on dry ice and stored at -25 to -15℃. Shelf life: 2 years.
3. Upon receipt, please check that all 7 components are included and immediately store them at their respective temperatures.
Application
Cell species identification
Figures
1. Broad Species Compatibility
Figure 1. Broad species compatibility. Clear amplification results were obtained across different species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6), and rhesus monkey (LLC-MK2).
Documents:
Safety Data Sheet
Manuals
Related Blog
One-Step Cell Identity Assurance: Species Authentication Made Simple
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