The Cell Species Identification Kit (Multiplex PCR Method) is designed for the identification of cell substrate origins, ensuring correct cell line identity and absence of cross-contamination from other cell lines. It enables quality control of raw materials in cell-based biopharmaceuticals.

This kit is based on multiplex PCR amplification of mitochondrial genes from animal cells, including cytochrome b, cytochrome c oxidase I (COX I), and cytochrome c oxidase II (COX II). Species origin is determined by analyzing the amplification products via agarose gel electrophoresis.

Features

• Broad Species Compatibility – Accurately detects 9 different cell species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6) and rhesus monkey (LLC-MK2).
• High Specificity – Reliably identifies contaminating cells even in the presence of primary cells (HEK293, MDCK, Vero, CHO).
• Reliable Results – Based on amplicon band number and size for clear interpretation.
• High Quality & Stable Supply – Ensures consistent performance and reliable availability.

Specifications

Components

Storage

This Product should be stored at -25°C ~ -15°C. Valid for 2 years.

Application

Cell species identification

Figures

1. Broad Species Compatibility

Figure 1. Broad species compatibility. Clear amplification results were obtained across different species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6), and rhesus monkey (LLC-MK2). 

Documents:

Safety Data Sheet

41341_MSDS_HB250904_EN.PDF

Manuals

41341_Manual_Ver.EN20250901.pdf

Related Blog

One-Step Cell Identity Assurance: Species Authentication Made Simple