Exosomes are small extracellular vesicles (30–150 nm in diameter) secreted by living cells, characterized by a typical lipid bilayer structure. They are present in cell culture supernatants, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry a variety of proteins, lipids, DNA, and RNA, playing critical roles in intercellular communication and material transfer. They also hold great promise as early diagnostic biomarkers for various diseases.

This product is a specialized serum-free medium developed specifically for exosome production. Its main components include HEPES, L-Glutamine, Pharmaceutical-grade Albumin, Hypoxanthine, Thymidine, Phenol Red, and Glucose. It is xeno-free, serum-free, and free of growth factors. Cells can be maintained in a fully supportive state without the addition of serum, facilitating high-quality exosome harvest.

Features

Serum-free & exosome-depleted → low background

Boosts exosome yield without compromising cell health

Chemically defined & ready-to-use

Broad compatibility: tumor cells, T cells

Ideal for omics & diagnostic applications

Components

Storage

This product should be stored at 2~8℃ for 1 year. Protect from light.

Application

Exosome Cultivation: Tumor Cell Lines & T Cells

Instructions

1. Cell recovery and passaging: After thawing, culture cells in complete medium for 2–3 passages until normal growth is confirmed.

2. Stepwise adaptation – Step 1: When cells reach ~50% confluency, remove the original complete medium and replace with a mixture of 50% exosome-specific serum-free medium + 50% complete medium.

3. Stepwise adaptation – Step 2: Once cells in Step 1 reach 70–80% confluency, remove the 50:50 medium mixture.

4. Wash cells 2–3 times with 1× PBS.

5. Add exosome-specific serum-free medium and continue incubation for 24–48 hours. Collect the cell culture supernatant when confluency reaches 90–100%. This supernatant is suitable for downstream exosome isolation.

Recommended Medium Volumes

Figures

1. Colony Formation Assay (HeLa cells):

Group A: Complete medium

Group B: Exosome-specific serum-free medium

2. Additional TEM Comparison:

Group A: Exosome-depleted serum-containing medium

Group B: Standard serum-containing medium

Group C: Exosome-specific serum-free medium

3. NTA (Nanoparticle Tracking Analysis) Results (HeLa cells)

Documents:

Safety Data Sheet

41210_MSDS_HB251216_EN.PDF

Manuals

41210_Manual_Ver.EN20251216.pdf