The Hieff Trans™ UltraProFect Transfection Reagent is a highly efficient transfection reagent designed specifically for protein expression, primarily used for transient transfection and protein production in CHO and 293 suspension systems. It combines the powerful capability of rapid, ultra-high yield transient protein production in suspension culture with the exceptional advantages of CHO and 293 cell expression systems. This transfection reagent is meticulously designed for high-density CHO and 293 suspension cultures, significantly enhancing protein expression outcomes.

In various CHO cell lines (including expiCHO, CHO-S, CHO-K1, etc.) and 293 cell lines (expi293, 293F, etc.), this transfection reagent achieves highly efficient protein expression. It meets the preparation needs of complex proteins such as antigen proteins, cytokines, monoclonal antibodies, nanobodies, and bispecific antibodies. It significantly improves expression efficiency and yield, fulfilling protein production requirements across diverse application scenarios.

Features

Broader Compatibility

Supports both CHO and 293 transient expression systems.

Superior Yield

Delivers approximately 2-fold higher protein expression yields compared to leading brands.

Versatile Applications

Capable of producing a wide range of complex proteins, including antigen proteins, cytokines, monoclonal antibodies, nanobodies, and bispecific antibodies.

Specifications

Components

Figure

Case Studies: Transient Expression in CHO Cell Lines

(A) . Monoclonal Antibody Expression Comparison

(B) . Nanobody Expression Comparison

(C). Bispecific Antibody(BsAb) Expression Comparison

Figure 1. Performance comparison of Yeasen’s CHO Protein Expression Kit vs. leading brands in antibody yield, cell viability, and cell density.

Cell line: ExpiCHO, initial transfection density: 6×10⁶ cells/mL. Expression monitored over 7 days (D2-D7). Yeasen’s Hieff Trans™ UltraPRO-CHO Transfection Reagent Kit achieves comparable antibody titers, cell viability, and cell growth profiles to leading brand reagents across full-length monoclonal antibodies, nanobodies, and bispecific antibodies, demonstrating robust and reliable performance for high-yield protein production.

Case Studies: Transient Expression in 293 Cell Lines

(A). Antigen-Fc Expression Comparison

(B). Antigen-His Expression Comparison

(C). Antibody Expression Comparison

Figure 2. Performance comparison of Yeasen’s UltraPRO-293 Transient Transfection Kit vs. leading brands in antibody and antigen expression.

Cell line: Expi293. Initial transfection density: 4×10⁶ cells/mL. Expression monitored over 7 days (D3-D7). Yeasen’s Hieff Trans™ UltraPRO-293 Transient Transfection Kit enables efficient expression of antibodies, antigen-Fc, and antigen-His proteins, delivering 2–3 times higher protein yield than leading brands while meeting diverse application needs.