This product is a high-performance cationic polymer–based transfection reagent, designed for efficient plasmid DNA transfection in a wide range of adherent and suspension cell types. It is particularly optimized for HEK293 cells (both suspension and adherent cultures), where it demonstrates outstanding performance in protein expression and viral packaging applications. The reagent can significantly increase AAV and other viral vector yields while effectively improving viral particle integrity.

Carefully formulated and optimized, this reagent features a defined molecular structure and uniform molecular weight, ensuring consistent and highly efficient transfection results across experiments. Its unique formulation promotes the rapid formation of stable polymer–DNA complexes and facilitates efficient intracellular release of DNA, enabling excellent transfection efficiency with minimal cytotoxicity. This makes it an ideal and reliable choice for various cell-based studies requiring high transfection performance and reproducibility.

Features

High Efficiency:

Provides superior transfection efficiency in a wide variety of cell lines, especially in HEK293 cells, enabling robust gene expression.

Low Cytotoxicity:

Maintains excellent cell viability and morphology after transfection, minimizing experimental variability.

Supports Multi-Plasmid Transfection:

Optimized for co-transfection of multiple plasmids, ideal for AAV/LV viral vector production and transient recombinant protein expression.

Chemically Defined and Animal-Free:

Fully synthetic formulation without animal-derived ingredients, ensuring high purity and consistent performance.

Easy to Use and Scalable:

Simple, ready-to-use protocol compatible with both small-scale research and large-scale production workflows.

Reproducible Results:

Uniform molecular composition and batch-to-batch consistency guarantee reliable transfection outcomes every time.

Components

Shipping and Storage

This product should be stored at 2~8℃ for 12 months.

Application

Cell Transfection; DNA Transfection; mRNA/SiRNA Transfection; AAV Manufacturing.

Figures

1. High cell transfection efficiency

Figure 1. High-efficiency DNA transfection in common cell lines.

HEK 293T and MCF-7 cells were transfected with a GFP-expressing plasmid using Hieff Trans™ PEI. Strong green fluorescence indicates efficient DNA delivery and expression 24–48 h post-transfection.

2. Application in AAV transfection

Figure 2. Efficient AAV2 and AAV5 production using Hieff Trans™ PEI transfection reagent.

For AAV2 production, HEK 293 cells were transfected with Hieff Trans™ PEI at an optimized DNA-to-reagent ratio of 1 µg : 2 µL in a 1 L flask, yielding approximately 1.8 × 10¹¹ vg/mL.

For AAV5 production, suspension HEK 293F cells were transfected at DNA-to-reagent ratios ranging from 1 µg : 1 µL to 1 µg : 3 µL, achieving virus particle yield, vector genome titer, and VG/VP ratios comparable to brand P*.

Documents:

Safety Data Sheet

40819_MSDS_HB251110_EN.PDF

Manuals

40819_Manual_Ver.EN20251110.pdf