The Hieff™ Endo-free Plasmid Midi Kit enables rapid purification of 100–500 µg of high-purity, high-copy plasmid DNA from 30–70 mL of E. coli LB (Luria-Bertani) culture. The spin column utilizes a proprietary silica-based matrix developed by our company, combined with a unique lysis buffer formulation, to maximize recovery of high-quality plasmid DNA. A specialized endotoxin-removal process ensures extremely low endotoxin levels (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The purified plasmid is also directly suitable for a wide range of downstream molecular biology applications, including restriction digestion, transformation, PCR, in vitro transcription, sequencing, and more.

Features

High Stability: Specially designed silica membranes in spin columns ensure minimal variation between columns and excellent reproducibility.

High Yield: Quickly and easily extract 100–500 µg of high-copy plasmid DNA from 30–70 mL E. coli LB culture.

Low Endotoxin: Unique formulation reduces endotoxin to <0.1 EU/µg DNA, ideal for efficient cell transfection.

Components

Storage

Part I: Store at –25°C to –15°C

Part II: Store at 2–8°C

Part III: Store at room temperature

Valid life: 2 years

Documents:

Safety Data Sheet

19023_MSDS_HB251212_EN.PDF

Manuals

19023_Manual_Ver.EN20251212.pdf