설명
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl termini of adjacent nucleotides in double-stranded DNA or RNA with either cohesive or blunt ends. This reaction requires ATP as a cofactor. Additionally, T4 DNA Ligase can repair single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids.
Specifications
|
Cat.No. |
14966ES40 / 14966ES42 |
|
Size |
40 KU / 400 KU |
|
Concentration |
400 U/μL |
|
Source |
Recombinant E. coli strain carrying the T4 DNA ligase gene. |
|
Enzyme Activity Definition |
One unit is defined as the amount of DNA ligase required to achieve 50% ligation of 100 ng of cohesive-end DNA fragments in 50 µL of 1X DNA Ligase Reaction Buffer with incubation for 30 minutes at 23°C. |
Components
|
Components No. |
Name |
14966ES40 |
14966ES42 |
|
14966-A |
Premium T4 DNA Ligase (400 U/μL) |
100 µL |
1 mL |
|
14966-B |
2 × Rapid Ligation Buffer |
1 mL |
10 mL |
|
14966-C |
10 × T4 DNA Ligase Buffer |
1 mL |
10 mL |
[Note]:
① High-concentration Premium T4 DNA Ligase combined with 2× Rapid Ligation Buffer significantly enhances the speed and efficiency of blunt-end ligation. Therefore, prolonged incubation (>10 minutes) is not recommended, as it may substantially reduce the transformation efficiency of the ligation products.
② The 10× T4 DNA Ligase Buffer does not contain PEG and is compatible with standard ligation protocols that do not specify the use of rapid ligation buffer.
Storage
This product should be stored at -25~-15℃ for 2 years.
Notes
1. For your safety and health, wear a lab coat and disposable gloves while handling this product.
2. This product is intended for research use only.
3. It is normal for a small amount of precipitate to appear when the buffer is thawed. Simply invert and mix well before use.
Documents:
Safety Data Sheet
Manuals
결제 및 보안
귀하의 결제 정보는 안전하게 처리됩니다. 당사는 신용카드 정보를 저장하지 않으며 귀하의 신용카드 정보에 접근할 수 없습니다.
문의
당신은 또한 좋아할 수 있습니다
자주 묻는 질문
이 제품은 연구 목적으로만 사용되며 인간이나 동물의 치료 또는 진단용으로 의도되지 않았습니다. 제품과 콘텐츠는
특정 애플리케이션에는 추가적인 제3자 지적 재산권이 필요할 수 있습니다.
예슨은 윤리적 과학에 헌신하며, 우리의 연구가 안전과 윤리적 기준을 보장하는 동시에 중요한 문제를 해결해야 한다고 믿습니다.