The CpG cytosines on the sequence of Hieff™ Methylated PUC19 DNA are fully methylated, which can be used as a known "positive" standard to evaluate the efficiency and accuracy of the experimental workflow. After sequencing, theoretically the C on CpG will not be converted. By calculating how many C have been converted to T, the over-conversion efficiency of bisulfite in this experiment can be evaluated, ensuring the reliability of subsequent analysis. The full length of the PUC19 DNA sequence is 2686 bp.

Specifications

Storage

The product should be stored at -25°C to -15°C for 2 years.

Notes

1. As a "spike-in" (positive control), it is recommended to spike into real samples at a ratio of 0.1%–1% and proceed with the entire experimental workflow together.

2. For your safety and health, please operate with lab coats and disposable gloves.

3. This product is for research use only.

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