GoldBand™ DL600 DNA Marker _ 10502ES

Yeasen제품번호: 10502ES60

Size: 500 μL+1 mL
가격:
판매 가격$55.00
재고:
재고 있음

The GoldBand™ DL600 DNA Marker consists of six linear double-stranded DNA fragments of 600 bp, 500 bp, 400 bp, 300 bp, 200 bp, and 100 bp, stored in 1× DNA Loading Buffer, ready for direct use in electrophoretic analysis. The indicator band is 400 bp, with a concentration of 100 ng/5 μL, while all other bands are 40 ng/5 μL. This product is suitable for the analysis of DNA bands in agarose gel electrophoresis and is not recommended for polyacrylamide gel electrophoresis.

Features

Superior Performance: Clean background, stable bands, and precise sizing.

Semi-Quantitative: Indicator bands with known concentrations for easy analysis.

Ready-to-Load: Pre-mixed with loading buffer for direct electrophoresis.

Room Temperature Stability: Stable for 6 months at RT.

Bonus: Includes 5× loading buffer for your samples.

Components

Components No.

Name

10502ES60

10502ES80

10502-A

GoldBand DL600 DNA Marker

500 μL

500 μL×10

10502-B

5×DNA Loading buffer

1 mL

1 mL×10

Electrophoresis Diagram

Figure 1. Electrophoretic Band Pattern of GoldBand DL600 DNA Marker

Figure 1. Electrophoretic Band Pattern of GoldBand DL600 DNA Marker

Storage

Store at room temperature or 2~8 ° C, valid for 6 months; store at -25~-15 ° C, valid for 2 years. Avoid repeated freeze-thaw cycles.

FAQ

It is a mixture of PCR products.
Simply ensure that the size of your target band falls within the range of the smallest and largest bands of the DNA Marker for accurate size reference.
This Marker is designed for agarose gel electrophoresis. It has not been tested in PAGE gels.
It depends on the gel type. For denaturing gel electrophoresis, using a DNA Marker is not recommended as the double strands will separate. For standard agarose gel electrophoresis, a DNA Marker is not accurate for determining RNA size, but it is acceptable for a rough estimation.
Possible causes: a) Voltage is too high; b) YeaRed nucleic acid stain was added when the agarose was still too hot.
Solutions: Adjust the voltage to 110-130V and run for at least 45 minutes. Allow the melted agarose to cool until it is no longer hot to the touch before adding the YeaRed stain.
This is likely due to sample overloading.
Solution: Reduce the sample loading volume.
This is a normal phenomenon. Cationic (positively charged) nucleic acid stains migrate in the opposite direction to nucleic acids in an electric field. Since small DNA fragments migrate faster and are ahead of the stain, they bind less dye, resulting in weaker fluorescence.

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