New Arrival: ATP Vitality Detection Kit with More Stable Signal

In the vast field of biomedical research and application, cell viability detection has always been a focal point for researchers and clinical workers. The vitality state of cells directly affects the accuracy of experimental results, the effectiveness of drug screening, and the reliability of disease diagnosis.

ATP is the fundamental source of cellular energy and plays a crucial role in various physiological processes within cells. It is an important indicator of cellular metabolism. When cells die, ATP rapidly hydrolyzes. Therefore, there is a good linear relationship between ATP content and the number of living cells.

ATP can be quantified by measuring the intensity of chemiluminescence through the ATP-dependent luciferase-catalyzed luciferin luminescent reaction. Since ATP content can well reflect the number of living cells and there is a direct proportionality between ATP content and chemiluminescence intensity, it is simple to calculate cell viability or cell numbers through the intensity of chemiluminescence.

Yeasen is proud to introduce the ATP Luminescent Cell Viability Detection Kit 2.0 (Cat#40211ES). Compared with similar products, this kit has particularly stable luminescence signals. Within one hour, the signal fluctuation range is usually no more than ±10%, with a half-life as long as 3 hours. Moreover, the storage stability is excellent—the kit can maintain over 90% activity when stored at room temperature for 30 days or at 2-8℃ for 60 days.

Product Features

1. Wide Linear Range: In a 96-well plate, there is a good linear relationship between 100 and 20,000 cells, although the upper limit of detectable cell numbers may vary for different cell types.

2. Convenient to Use: The kit is easy to operate. The reagents provided in the kit are ready-to-use. The readings are stable, and the detection speed is fast. The entire detection process takes only about 10 minutes. There is no need to wash the cells or replace or remove the culture medium.

3. Excellent Stability: With a half-life as long as 3 hours, the kit can maintain over 90% activity when stored at room temperature for 30 days or at 2-8℃ for 60 days.

Product Performance

Figure 1. The product's fluorescence values are similar to those of competitors

Figure 2. The product have a good linear relationship within 100,000 cells.

Figure 3.The fluorescence values remain stable even after 5 hours of incubation.

Figure 4.The product shows a stability decline of less than 10% after being stored at 4℃ for 60 days or at room temperature (24.5℃) for about 30 days

Product Code	40206ES	40203ES	40210ES/40211ES	40209ES	40205ES	40208ES
Name	MTT Cell Proliferation Assay Kit	Cell Counting Kit (CCK-8)	ATP Luminescent Cell Viability Assay Kit	LDH Cytotoxicity Assay Kit	SRB Cell Proliferation and cytotoxicity assay kit	Trypan Blue Staining Cell Viability Assay Kit
Detection Sensitivity	++	+++	++++	+++	++	+++
Detection Time	Longer	Shorter	Extremely Short	Shorter	Longer	Short
Detection Indicator	Dehydrogenases in the mitochondria of living cells		ATP	LDH released from lysed cells into the culture medium	Intracellular basic amino acids	Dehydrogenases in the mitochondria of living cells
Detection Wavelength	A560-600nm	A420-480nm	Chemiluminescence	A490nm	A515nm	A570
Cytotoxicity	High,	Low	Cells will be lysed	Only detects cell supernatant	Cells need to be fixed	Low,
Reagent Stability	+	++++	++++	++	++++	+++
Ease of Use	+	++++	++++	++	+++	++
Direct Detection of Cell Proliferation	No	No	No	No	No	No

Cell Proliferation Detection------Fluorescent Dye Method

Product Code	Name	Ex/Em nm	Staining Location	Application
40757ES	DIR Iodide (DiIC18(7))	748/780	Cell membrane staining, deep red fluorescence	Live cell staining, in vivo imaging or tracing experiments, exosome staining
40725ES	DIO (DIOC18(3))	484/501	Cell membrane staining, green fluorescence	Live cell staining, in vivo imaging
40758ES	DID Perchlorate (DiIC18(5))	644/663	Cell membrane staining, red fluorescence	Extracellular matrix staining, live cell staining, staining of cells or tissues with other fluorescence
40714ES	CFDA SE Cell Proliferation and Cell Tracking Kit	494/521	Cell membrane staining, green fluorescence	Cell proliferation analysis, in vivo proliferation research
40721ES	Live Cell Tracer CMFDA (Green)	492/517	Cell proliferation, green fluorescence	Cell proliferation, cell fusion, cell adhesion, and multidrug resistance transport protein research, pathogen labeling
40792ES	Live Cell Tracer CM-DiI (Red)	548/563	Cell membrane staining, red fluorescence	Live animal staining, cell staining
40747ES	Calcein-AM/PI Live/Dead Cell Double Staining Kit	490/515	Calcein-AM: Live cell cytoplasm staining, green fluorescence; PI: Dead cell nucleus staining, red fluorescence	Live cell/dead cell staining
40711ES	PI (Propidium Iodide)	535/617	Cell nucleus DNA	Dead cell staining
40745ES	7-AAD Viability Staining Solution	546/647	Cell nucleus DNA	Dead or late apoptotic cell staining
40202ES	Alamar Blue	560/590	In oxidized state, it appears purple-blue without fluorescence; in the proliferative phase of cells, it is reduced to a pink or red fluorescent product	Proliferation of animals, fungi, and bacteria

Ordering Information

Product Positioning	Product Name	Product Code	Product Specification
Cell Proliferation Detection	ATP Luminescent Cell Viability Detection Kit 2.0	40211ES10	10 mL
		40211ES60	100 mL
		40211ES70	10×100 mL