説明
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene .Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).
Specifications
|
Cat.No. |
96273ES25 / 96273ES60 / 96273ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96273ES25 |
96273ES60 |
96273ES76 |
|
Recombinant Cynomolgus MSLN/Mesothelin Protein, His Tag, Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
MSLN;CAK1 antigen;CAK1;Mesothelin;MPF;MPFSMRP;SMR; |
|
Species |
Cynomolgus |
|
Expression Sequence |
Asp296-Ser598 |
|
Accession |
XP_005590873.3 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 34.87 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
ApplicationCase
ELISA Data 1: Immobilized Cynomolgus MSLN, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 0.12 μg/mL determined by ELISA.
ELISA Data 2: Immobilized Cynomolgus MSLN, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.8 ng/mL determined by ELISA.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
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