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LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation . Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain.
Specifications
|
Cat.No. |
96271ES25 / 96271ES60 / 96271ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96271ES25 |
96271ES60 |
96271ES76 |
|
Recombinant Cynomolgus LILRA4/CD85g Protein,His Tag,Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
CD85g;ILT7;ILT-7;ILT7MGC129598;LILRA4;MGC129597 |
|
Species |
Cynomolgus |
|
Expression Sequence |
Glu24-Asn446 |
|
Accession |
XP_005590346.1 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
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