Recombinant Human PDGF-AB Protein

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Recombinant Human PDGF-AB Protein _ 91618ES

Features

1. Complete FDA DMF certification (MF038306)
2. GMP-grade, no animal-derived ingredients, strict quality control and production in strict accordance with GMP standards to ensure the introduction of no animal-derived ingredients.
3. High enzyme cutting efficiency, low star activity, and good lethality. It has high enzyme cutting efficiency and no star activity after 16 hours of reaction at 37°C. It has good lethality after enzyme digestion-ligation-re-digestion test.
4.Superior performance application performance is comparable to competing products

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Bsa I GMPグレード（20 U/μL）_ 10661ES

Yeasen

SKU: 10661ES03

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Price:

$175.00

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0 in stock

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PDGF-AB (Platelet-derived growth factor-AB) is an important member of the platelet-derived growth factor (PDGF) family. It belongs to heterodimeric proteins, formed by the dimerization of PDGFA and PDGFB subunits. It is a class of extracellular signaling molecules with diverse biological activities, widely involved in various physiological and pathological processes of the body. The structural core of PDGF-AB consists of two subunits (α and β) linked by disulfide bonds to form a stable protein structure. Distinct from the homodimers of the PDGF family (such as PDGF-AA and PDGF-BB), its structural characteristics determine the uniqueness of its receptor binding specificity and biological functions. It can specifically bind to PDGF receptors (PDGFR) on the cell surface and initiate downstream signaling pathways.

This product is provided as a lyophilized powder, featuring high activity, high purity, low endotoxin, high stability, and no tag.

Product Information

Product Information.	Catalog Number	Size
Recombinant Human PDGF-AB Protein	91618ES10	10 μg
	91618ES50	50 μg
	91618ES80	1 mg

Performance Parameters

Synonyms	Platelet-derived Growth Factor AB; PDGFAB
Species	Human
Source	CHO Cells
Sequence	Human PDGF-A(Ser87-Thr211)&Human PDGF-B(Ser82-Thr190)
Accession	P04085&P01127
Tag	Tag Free
Molecular Weight	Predicted molecular mass：14.3 kDa&12.3 kDa
Endotoxin	≤10 EU/mg by the LAL method
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Activity	Measure in a cell proliferation assay using NIH3T3 cells. The ED50 is ≤1.0 ng/mL.
Formulation	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Appearance	Lyophilized Powder

Storage

Store at -25~-15°C. Valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C for 7-10 days. After reconstitution, store at -85~-65°C for up to 6 months.

Reconstitution Method

Reconstitute the lyophilized protein with sterile deionized water. It is recommended to aliquot the protein upon first use to avoid repeated freeze-thaw cycles.

Notes

1. For your safety and health, please wear a lab coat and disposable gloves during operation.

2. This product is for research use only.

Documents:

Manuals

91618_Manual_HB20260408

Citation

Discription:

This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and provided in a liquid form.

Applications

Specifications

Expression Host

Recombinant E. coli with Bas I gene

Reaction Temperature

37℃

Storage Buffer

10mM Tris-HCl, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.2mg/ml OsrHSA pH 7.4±0.2 (25℃)

Unit Definition

1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system.

Application

1.Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);

2.Digestion of DNA to obtain specific sticky ends；

3.Linearize plasmid template before in-vitro transcription.

Components

Components No.	Name	10661ES03	10661ES10	10661ES60
10661	Bsa I GMP-grade (20 U/μL)	(1 KU) 50 μL	(10 KU) 500 μL	(100 KU) 5 mL

Shipping and Storage

This product should be stored at -25 ~ -15℃ for two years.

Description

10661_1_1_1024x1024__PID:92709aa9-3249-4916-800b-042b54e3aed9

Figure1. No non-specific nuclease residues test

20 U BsaI was incubated with substrate DNA at 37°C for 1 h and 16 h, and DNA band changes were compared by agarose gel electrophoresis. The results showed that there was no non-specific nuclease residue in BsaI.

10661-2_1_1_1024x1024__PID:870034f6-32c2-42fd-898b-dff92eca1e3c

Figure2. The end integrity is good and the effect is consistent with imported brands

When BsaI and substrate DNA were incubated in the enzyme digestion reaction system for 16 hours at 37°C, no non-specific degradation of the substrate caused by star activity was detected, indicating that there was no star activity after 16 hours of incubation with BsaI.