Cat.No.

91349ES08 / 91349ES20 / 91349ES60 / 91349ES76

Size

5 μg / 20 μg / 100 μg / 500 μg

Name

91349ES08

91349ES20

91349ES60

91349ES76

HiActi™ Recombinant Human FGF-3 Protein, His Tag

5 μg

20 μg

100 μg

500 μg

Synonyms

fibroblast growth factor 3, HBGF-3, INT2, FGF3

Species

Human

Expression Sequence

MDAGGRGGVYEHLGGAPRRRKLYCATKYHLQLHPSGRVNGSLENSAYSILEITAVEVGIVAIRGLFSGRYLAMNKRGRLYASEHYSAECEFVERIHELGYNTYASRLYRTVSSTPGARRQPSAERLWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLDHRDHEMVRQLQSGLPRPPGKGVQPRRRR with polyhistidine tag at the C-terminus.

Accession

P11487

Tag

C-His

Expression System

E.coli

Molecular Weight

The protein has a calculated MW of 21.99 kDa. The protein migrates as 22 kDa under reducing condition

Purity

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4

Appearance

Lyophilized Powder

Biological activity

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <78 ng/mL.

Reconstitution Instructions

1)Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening.

2)It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution.

3)The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier.