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Tumor necrosis factor-alpha (TNF-α) is a pleiotropic pro-inflammatory cytokine secreted by various cell types, including adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. It belongs to the TNF ligand family and signals through two receptors, TNFR1 and TNFR2. TNF-α exhibits cytotoxicity against various tumor cells and is a key mediator of the immune response against bacterial infections. TNF-α is also involved in the induction of inflammatory responses, regulation of cell proliferation, differentiation, and apoptosis.
Product Information
|
Product Name |
Catalog No. |
Size |
|
Recombinant Human TNF-alpha Protein |
90635ES10 |
10 μg |
|
90635ES50 |
50 μg |
|
|
90635ES60 |
100 μg (2x50 μg) |
|
|
90635ES82 |
1 mg |
Product Properties
|
Synonyms |
Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF |
|
Species |
Human |
|
Expression Sequence |
Val77-Leu233 |
|
Accession |
P01375 |
|
Tag |
No Tag |
|
Expression System |
E. coli |
|
Molecular Weight |
Approximately 17.5 kDa |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC |
|
Endotoxin |
< 0.01 EU per μg by the LAL method |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 10 mM NaCl, pH 7.0 |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml. |
|
Reconstitution |
1. Briefly centrifuge the vial before opening to bring the contents to the bottom. 2. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/ml. 3. Further dilutions may be prepared for subsequent experiments; for long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. |
Feature
- High purity
- High activity
- Llow endotoxin
- High batch-to-batch consistency
Application
SDS-PAGE
Figure 1. Human TNF-α GMP Protein on SDS-PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
Figure 2. The purity of Human TNF-α GMP Protein is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
Figure 3. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml.
Storage
Store at -25~-15°C. Valid for 1 year upon receipt. After reconstitution, under sterile conditions, store at 2~8°C, valid for 7 days. For long-term storage, aliquot and store at -85 to -65°C under sterile conditions for up to 3 months.
Documents:
Safety Data Sheet
Manuals:
Storage
FAQ
Citations
Documents
Applications
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