Cat.No.

90294ES08 / 90294ES20 / 90294ES60 / 90294ES76

Size

5 μg / 20 μg / 100 μg / 500 μg

Name

90294ES08

90294ES20

90294ES60

90294ES76

HiActi™ Recombinant Mouse IL-28B Protein ,His Tag

5 μg

20 μg

100 μg

500 μg

Synonyms

interleukin 28B, interferon lambda 3, IFNL3, IFN-lambda-3, IFN-lambda-4, IL28B

Species

Mouse

Expression Sequence

MVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV with polyhistidine tag at the C-terminus.

Accession

Q8IZI9

Tag

C-His

Expression System

E.coli

Molecular Weight

The protein has a calculated MW of 25.57 kDa. The protein migrates as 21 kDa under reducing condition

Purity

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.

Appearance

Lyophilized Powder

Biological activity

Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <5 ng/mL.

Reconstitution Instructions

Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening.

It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution.

The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier.