説明
The Cell Species Identification Kit (Multiplex PCR Method) is designed for the identification of cell substrate origins, ensuring correct cell line identity and absence of cross-contamination from other cell lines. It enables quality control of raw materials in cell-based biopharmaceuticals.
This kit is based on multiplex PCR amplification of mitochondrial genes from animal cells, including cytochrome b, cytochrome c oxidase I (COX I), and cytochrome c oxidase II (COX II). Species origin is determined by analyzing the amplification products via agarose gel electrophoresis.
Features
- Broad Species Compatibility – Accurately detects 9 different cell species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6) and rhesus monkey (LLC-MK2).
- High Specificity – Reliably identifies contaminating cells even in the presence of primary cells (HEK293, MDCK, Vero, CHO).
- Reliable Results – Based on amplicon band number and size for clear interpretation.
- High Quality & Stable Supply – Ensures consistent performance and reliable availability.
Specifications
|
Cat NO. |
41341ES25 / 41341ES50 |
|
Size |
25 T / 50 T |
Components
|
Components No. |
Name |
41341ES25 |
41341ES50 |
|
41341-A |
2× Multiplex PCR Buffer |
312.5 μL |
625 μL |
|
41341-B |
Primers Mix |
112.5 μL |
225 μL |
|
41341-C |
Cell Extraction Buffer |
1.25 mL × 2 tubes |
1.25 mL × 4 tubes |
|
41341-D |
Proteinase K (20 mg/mL) |
50 μL |
100 μL |
|
41341-E |
DL500 DNA Marker |
125 μL |
250 μL |
|
41341-F |
DEPC-Treated Water |
1.25 mL |
1.25 mL × 2 tubes |
|
41341-G |
Positive Control |
25 μL |
25 μL × 2 tubes |
Storage
This Product should be stored at -25°C ~ -15°C. Valid for 2 years.
Application
Cell species identification
Figures
1. Broad Species Compatibility
Figure 1. Broad species compatibility. Clear amplification results were obtained across different species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6), and rhesus monkey (LLC-MK2).
Documents:
Safety Data Sheet
Manuals
41341_Manual_Ver.EN20250901.pdf
Related Blog
One-Step Cell Identity Assurance: Species Authentication Made Simple
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この製品は研究目的のみに使用され、人間や動物の治療や診断に使用することを意図したものではありません。製品とコンテンツは、
特定のアプリケーションでは、追加のサードパーティの知的財産権が必要になる場合があります。