Enterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme that initiates protein digestion in the intestine. It precisely activates trypsinogen to trypsin, which then cascade-activates chymotrypsinogen and procarboxypeptidase. Its expression is specifically localized in differentiated intestinal cells and goblet cells, making it both a core regulator of nutritional digestion and a reliable marker of intestinal epithelial maturation.
The Enterokinase Activity Assay Kit is a rapid method for determining enterokinase activity in biological samples. This method detects enterokinase activity by cleaving a 7-amino-4-trifluoromethylcoumarin (AFC)-labeled peptide substrate containing an enterokinase recognition sequence. The intensity of the fluorescent product AFC (excitation wavelength Ex=380 nm, emission wavelength Em=500 nm) generated by the reaction is proportional to the enzyme activity in the sample.
Product Information
|
Component No. |
Component Name |
20393ES60 (100 T) |
|
20393-A |
200× Enterokinase Substrate |
24 μL |
|
20393-B |
1 mM AFC Standard |
100 μL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Properties
|
Cat# |
Component Name |
20393ES60 (100T) |
|
20393-A |
200x Enterokinase Substrate |
24 uL |
|
20393-B |
1 mM AFC Standard |
100 uL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Figures
Figure 1. AFC standard curve.
Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions.
Storage
FAQ
Citations
Documents
| Brochure |
|---|
| Industrial_Production_Solutions_for_Tirzepatide_and_Semaglutide.pdf |
Applications
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