説明
HieffTM Endo-free Plasmid Mini Kit is designed for rapid extraction of 20–50 µg of high-purity plasmid DNA from 1–10 mL of E. coli LB culture. This kit features high efficiency, speed, and convenience. The spin column utilizes a proprietary silica-based matrix material developed by our company. Combined with our unique lysis buffer formulation, it enables maximum recovery of high-purity DNA. The extracted DNA has high purity and extremely low endotoxin residue (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The DNA can be directly used in various molecular biology applications such as restriction digestion, transformation, PCR, in vitro transcription, and sequencing.
Feature
- Stable performance: Specially designed silica membranes ensure consistent DNA binding and reliable results.
- Fast and easy: Extract 20–50 µg high‑copy plasmid DNA from 1.5–5 mL E. coli LB culture in about 30 minutes.
- Pure DNA: Removes endotoxins to below 0.1 EU/µg DNA for excellent cell transfection.Specifications
Components
|
Category |
Components No. |
Name |
19021ES08 |
19021ES50 |
19021ES70 |
|
Part I |
19021-A |
RNase A (10 mg/mL) |
15 µL |
125 µL |
500 µL |
|
19021-B |
Endotoxin Removal Solution ER (ER Solution E2) |
500 µL |
5 mL |
20 mL |
|
|
Part II |
19021-C |
Buffer RS* (RS Buffer E2*) |
1.5 mL |
12.5 mL |
50 mL |
|
Part III |
19021-D |
Buffer AC (AC Buffer E2) |
500 µL |
5 mL |
20 mL |
|
19021-E |
DNA Binding Column E2 (HieffTM DNA Column E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-F |
2 mL Collection Tube (2 mL Collection Tube E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-G |
Lysis Buffer LB (LB Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-H |
Binding Buffer BD (BD Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-I |
Protein Lysis Buffer PL* (PL Buffer E2*) |
1.6 mL |
16 mL |
63 mL |
|
|
19021-J |
Wash Buffer W* (Wash Buffer*) |
1.3 mL |
13 mL |
50 mL |
|
|
19021-K |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Shipping and Storage
Store Part I components at -25°C ~ -15°C; store Part II components at 2~8°C;
store Part III components at room temperature. The product is valid for 1 year.
Application
plasmid DNA Extraction; Endo-free Plasmid Extraction; Plasmid preparation for transfection.
Figures
1. Superior plasmid quality with ultra‑low endotoxin
Figure 1. Plasmid DNA extracted using Yeasen 19021, Supplier O*, and Supplier T* kits was transfected into HEK293 cells with pEGFP‑C1. Bright‑field and green‑fluorescence images were taken at 72 h post‑transfection, showing transfection efficiency in the order Yeasen 19021> Supplier T* > Supplier O*.
Documents:
Safety Data Sheet
Manuals
19021_Manual_Ver.EN20250806.pdf
Citations & References:
[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)
[2] Zhang J, Wang W, Feng N, Jiang X, Zhu S, Chen YQ. Ndufa6 regulates adipogenic differentiation via Scd1. Adipocyte. 2021;10(1):646-657. doi:10.1080/21623945.2021.2007590(IF:4.534)
[3] Zhu S, Zhang J, Wang W, Jiang X, Chen YQ. Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis : Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis. J Physiol Biochem. 2022;78(2):377-388. doi:10.1007/s13105-022-00876-7(IF:4.158)
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