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This kit is designed for the isolation and purification of high-quality total RNA from a wide variety of sample types, including animal tissues (heart, liver, spleen, lung, muscle, fat, brain, etc.), cells, common plants, and polysaccharide‑ and polyphenol‑rich plant samples. A unique magnetic bead system combined with a carefully optimized buffer formulation efficiently captures the released nucleic acids, yielding RNA of high purity with minimal carry‑over of genomic DNA, protein and other impurities. The purified RNA is suitable for a broad range of downstream applications such as RT-PCR, real-time PCR, microarray analysis, Northern blot, dot blot, poly(A) selection, in vitro translation, RNase protection assay, and molecular cloning. The kit is fully compatible with YEASEN automated nucleic acid extraction instruments and the Thermo Scientific KingFisher Flex 96 and comparable systems. YEASEN offers integrated solutions for high‑throughput automated nucleic acid extraction.

Features

Broad sample compatibility: Works with various animal and plant tissues, including liver, kidney, spleen, heart, skin, aquatic samples, leaves, roots, fruits, and seeds.

Fast and simple: Extraction takes less than 1 hour per sample. Fully compatible with Yeasen 16/48/96-channel extraction instruments.

High-purity RNA: Includes DNase I for complete DNA removal. Ready for RT-PCR, library prep, and other downstream applications.

Components

Category	No.	Name	18613ES48
Part I	18613-A	DNase I	0.6 mL/tube × 1
Part II	18613-B	Proteinase K	1.1 mL/tube × 1
Part III	18613-C	Lysis Buffer AL	16 mL/bottle × 1
	18613-D	Lysis Buffer PL	50 mL/bottle × 1
	18613-E	Digestion Buffer DR	40 mL/bottle × 1
	18613-F	Binding Buffer RB	2 mL/bottle × 1. Add 28 mL isopropanol prior to first‑time use
	18613-G	Magnetic Bead Suspension	1.1 mL/tube × 1
	18613-H	Wash Buffer DW1	19 mL/bottle × 1. Add 19 mL absolute ethanol prior to first‑time use
	18613-I	Wash Buffer DW2	18 mL/bottle × 1. Add 72 mL absolute ethanol prior to first‑time use
	18613-J	Elution Buffer RE	5 mL/bottle × 1

Notes

1. If precipitation or turbidity appears in any reagent (common when stored at low ambient temperatures), warm to 30 °C and mix until fully dissolved.

2. Magnetic beads may carry over into the eluate. Avoid aspirating magnetic beads when transferring eluted RNA.

3. Avoid repeated freeze-thaw cycles of frozen samples, as this may compromise nucleic acid quality.

4. For your safety and health, wear a lab coat and disposable gloves when handling this product.

5. Several buffers contain guanidinium salts. Do NOT mix with oxidizing disinfectants such as sodium hypochlorite; toxic gas may form. Dispose of all kit‑related waste in accordance with local institutional regulations for biohazardous waste.

6. This product is for research use only.

Storage

Part I components should be stored at -25 to -15°C for 18 months.

Part II components should be stored at 2 to 8°C for 18 months.

Part III components should be stored at room temperature for 18 months.

FAQ

Citations

Documents

Manual
18613-Manual-Ver.EN20261026.pdf

Applications

よくある質問

法律および免責事項

この製品は研究目的のみに使用され、人間や動物の治療や診断に使用することを意図したものではありません。製品とコンテンツは、Yeasen Biotechnology が所有する特許、商標、著作権によって保護されています。商標記号は原産国を示しますが、必ずしもすべての地域で登録されているわけではありません。

特定のアプリケーションでは、追加のサードパーティの知的財産権が必要になる場合があります。

Yeasen は倫理的な科学に専念しており、私たちの研究は安全性と倫理基準を確保しながら重要な問題に取り組むべきだと考えています。