説明
The Hieff™ Pro Soil/Stool DNA Kit is designed to extract high-purity DNA from 250 to 500 mg of soil samples. Utilizing superparamagnetic bead purification technology, this method eliminates the need for toxic phenol-chloroform extraction, offering a simple and convenient workflow. The extracted DNA is ready for direct use in downstream applications such as PCR, qPCR, microarray analysis, and Sanger or Next-Generation Sequencing (NGS). When paired with automated magnetic bead extraction instruments, the kit enables high-throughput nucleic acid extraction.
Features
Broad Applicability: Compatible with 250–500 mg soil and fecal samples.
Deep Impurity Removal: Effectively eliminates complex inhibitors from challenging soil matrices.
High-Purity Nucleic Acids: Yields DNA ready for direct use in PCR, qPCR, and NGS/Third-Generation Sequencing.
High-Throughput Compatibility: Supports automated instruments for rapid, batch processing.
Components
|
Category |
Components No. |
Name |
18527ES48 (48 T) |
|
Part Ⅰ |
18527-A |
Proteinase K |
1.1 mL/tube × 1 |
|
Part II |
18527-B |
Magnetic Bead Suspension |
1.1 mL/tube × 1 |
|
18527-C |
Lysis Buffer |
30 mL/bottle × 1 |
|
|
18527-D |
Grinding Tube |
8 tubes/bag × 1 bag |
|
|
18527-E |
Lysis Enhancer SE |
3 mL/bottle × 1 |
|
|
18527-F |
Precipitation Solution |
7 mL/bottle × 1 |
|
|
18527-G |
Lysis Buffer LB |
30 mL/bottle × 1 |
|
|
18527-H |
Deproteinization Solution PL* |
40 mL/bottle × 1 |
|
|
18527-I |
Wash Buffer AW* |
40 mL/bottle × 1 |
|
|
18527-J |
Wash Buffer BW* |
40 mL/bottle × 1 |
|
|
18527-K |
Elution Buffer DE |
6 mL/bottle × 1 |
[Note]: Add 6.3 mL isopropanol into the bottle of 18527-H Deproteinization Solution PL.
Add 12 mL isopropanol into the bottle of 18527-I Wash Buffer AW.
Add 34 mL absolute ethanol into the bottle of 18527-J Wash Buffer BW.
Storage
Store Part I at 2–8°C, valid for 18 months.
Store Part II at room temperature, valid for 18 months.
Documents:
Safety Data Sheet
Manuals
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